Calcium independent pectinases with improved thermostability
AssigneeMetgen Oy
InventorsKlara Birikh, Anu Minna Maaret Suonpaa
Life of this patent
Estimating the term…
Abstract
The invention is in the field of protein chemistry, in particular in the field of enzymology. It provides pectinases, i.e. polypeptides with pectin-degrading properties. In particular the invention provides polypeptides with pectate lyase activity (EC 4.2.2.2). Enzymes according to the invention have improved properties, such as improved thermostability and decreased calcium dependence.
Claims21
A polypeptide with pectate lyase activity, the polypeptide comprising:
- an amino acid sequence that is at least 70% identical to the amino acid according to SEQ ID NO:
- 1, and
- a leucine residue at an amino acid position corresponding to position 231 in SEQ ID NO:
- 1.
A method for improving the thermostability of a polypeptide with pectate lyase activity comprising an amino acid sequence that is at least 90% identical to the amino acid according to SEQ ID NO:
- 1, the method comprising:
- altering the amino acid at a position corresponding to position 231 in SEQ ID NO:
- 1 to a leucine residue;
- wherein the polypeptide comprising a leucine residue at a position corresponding to position 231 in SEQ ID NO:
- 1 has an improved thermostability as compared to the pectate lyase of SEQ ID NO:
- 1.
A method for decreasing, abolishing or removing the calcium dependence of a polypeptide with pectate lyase activity comprising an amino acid sequence that is at least 90% identical to the amino acid according to SEQ ID NO:
- 1, the method comprising:
- altering the amino acid at a position corresponding to position 231 in SEQ ID NO:
- 1 to a leucine residue.
Dependencies are inferred from the claim text.