# US 11,008,550 B2 · Use of RNA for reprogramming somatic cells

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- Number: US 11,008,550 B2
- Document ID: US11008550B2
- Status: Granted
- Kind: B2
- Application number: 16/250,366
- Filed: 2019-01-17
- Published: 2019-06-27
- Granted: 2021-05-18
- Earliest claimed: 2007-12-14
- Assignees: BioNTech SE; TRON—Translationale Onkologie an der Universitätsmedizin der Johannes Gutenberg-Universität Mainz
- Applicants: BioNTech AG; TRON—Translationale Onkologie an der Universitatsmedizin der Johannes Gutenberg Universitat Mainz Gemeinnutzige GmbH
- Inventors: Ugur Sahin; Marco Poleganov; Tim Beissert
- Web page: https://patentgrep.com/patent/US11008550B2

## CPC

- C12N 5/0696: {Artificially induced pluripotent stem cells, e.g. iPS}
- A61P 43/00: Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
- C12N 2501/602: Sox-2
- C12N 2501/603: Oct-3/4
- C12N 2501/604: Klf-4
- C12N 2501/606: c-Myc
- C12N 2510/00: Genetically modified cells
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

## Abstract

The present invention provides methods for de-differentiating somatic cells into stem-like cells without generating embryos or fetuses. More specifically, the present invention provides methods for effecting the de-differentiation of somatic cells to cells having stem cell characteristics, in particular pluripotency, by introducing RNA encoding factors inducing the de-differentiation of somatic cells into the somatic cells and culturing the somatic cells allowing the cells to de-differentiate.

## Claims

### Claim 1

A RNA-transfected somatic cell population which comprises somatic cells subjected to electroporation, containing in vitro transcribed mRNA encoding OCT4, SOX2, KLF4 and c-MYC, wherein the 5′-Cap of said mRNA consists of the D1 isomer of m27,2′-0 GppspG and wherein the transfected cell population expresses alkaline phosphatase and expresses endogenous OCT4, for at least 7 days.

### Claim 2 (depends on 1)

The RNA-transfected somatic cell population in accordance with claim 1 wherein the somatic cells are human cells.

### Claim 3 (depends on 2)

The RNA-transfected somatic cell population in accordance with claim 2 wherein the human cells are adult human dermal fibroblasts.

### Claim 4 (depends on 1)

The RNA transfected somatic cell population in accordance with claim 1 wherein the somatic cells are fibroblasts.