Compound for labeling amine-containing compounds, and its use

A method for analyzing N-glycans, amine-containing amino acids, amine-containing peptides, amine-containing proteins, or other amine-containing compounds in a sample, comprising:

• (i) contacting said N-glycans, amine-containing amino acids, amine-containing peptides, amine-containing proteins, or other amine-containing compounds in said sample with a labeling compound (“Activated Procaine”) with the structure
• or a solvate or a salt of said labeling compound,
• under conditions sufficient to create fluorescent, mass spectrometry-active derivatives of said N-glycans, of said amine-containing amino acids, of said amine-containing peptides, of said amine-containing proteins, or of said other amine-containing compounds in said sample,
• (ii) subjecting said fluorescent, mass spectrometry-active derivatives of said N-glycans, of said amine-containing amino acids, of said amine-containing peptides, of said amine-containing proteins, or of said other amine-containing compounds to chromatographic methods, thereby separating said fluorescent, mass spectrometry-active derivatives of said N-glycans, of said amine-containing amino acids, of said amine-containing peptides, of said amine-containing proteins, or of said other amine-containing compounds, and
• (iii) measuring fluorescence intensity of said separated fluorescent, mass spectroscopy-active derivatives of said N-glycans, of said amine-containing amino acids, said amine-containing peptides, of said amine-containing proteins, or of said other amine-containing compounds,
• thereby analyzing said N-glycans, amine-containing amino acids, amine-containing peptides, amine-containing proteins, or other amine-containing compounds in said sample.

A method of analyzing N-glycans present on a glycoprotein, said method comprising:

• (i) releasing said N-glycans from said glycoprotein as glycosylamines, thereby obtaining released glycosylamines,
• (ii) contacting said released glycosylamines with a labeling compound (“Activated Procaine”) with the structure
• or a solvate or a salt of said labeling compound,
• under conditions sufficient to create fluorescent, mass spectrometry-active derivatives of said released glycosylamines,
• (iii) subjecting said fluorescent, mass spectrometry-active derivatives of said released glycosylamines to chromatography to obtain separated fluorescent, mass spectrometry-active derivatives of said released glycosylamines, and
• (iv) measuring a fluorescent signal of said separated fluorescent, mass spectrometry-active derivatives of said released glycosylamines, thereby analyzing said N-glycans present on said glycoprotein.

A kit for labeling a compound of interest selected from the group consisting of an N-glycan, an amine-containing amino acid, an amine-containing peptide, an amine-containing protein, and another amine-containing compound in a sample, said kit comprising:

• (i) a container,
• (ii) a labeling compound, “Activated Procaine,” with the structure
• or a solvate or a salt of said labeling compound, said labeling compound being disposed in said container, and
• (iii) instructions for labeling said N-glycan, amine-containing amino acid, amine-containing peptide, amine-containing protein, or other amine-containing compound with said labeling compound.