Back to results
US 11,001,872 B2Granted
Designing customized protein-specific buffer system
OwnerShawn Clarkinventor
Life of this patent
Estimating the term…
Abstract
The present invention is related to the field of protein chemistry. In particular, mixed buffer compositions are formulated that allow an accurate identification of agent-induced changes in protein melting point temperatures. Such buffer compositions provide for methods that determine the specific effects of exogenous agents on protein stability, cryoprotective effects and/or protein quality control (e.g., synthesis and/or extraction purity validations).
Claims15
A device, comprising:
- a) a solid substrate comprising a plurality of testing wells and a control well, wherein each of said plurality of testing wells comprises a first buffer and a second buffer, said control well comprises a sample buffer, and each of said plurality of testing wells and control well comprises a protein sample;
- b) a first testing well series of said plurality of testing wells, wherein each well of said first testing well series comprises a different ammonium sulphate concentration;
- c) a second testing well series of said plurality of testing wells, wherein each well of said second testing well series comprises a different amino acid;
- d) a third testing well series of said plurality of testing wells, wherein each well of said third testing well series comprises a different urea concentration;
- e) a fourth testing well series of said plurality of testing wells, wherein each well of said fourth testing well series comprises a different glycerol concentration;
- f) a fifth testing well series of said plurality of testing wells, wherein each well of said fifth testing well series comprises a different hydrogen ion concentration;
- g) a sixth testing well series of said plurality of testing wells, wherein each well of said sixth testing well series comprises a different third buffer;
- h) a seventh testing well series of said plurality of testing wells, wherein each well of said seventh testing well series comprises a different dimethyl sulfoxide concentration;
- i) an eighth testing well series of said plurality of testing wells, wherein each well of said eighth testing well series comprises a different Hoefmeister Series compound;
- and
- j) a ninth testing well series of said plurality of testing wells, wherein each well of said ninth testing well series comprises an different metal ion.
8 more elements
A device, comprising:
- a) a solid substrate comprising a plurality of testing wells and a control well, wherein each of said plurality of testing wells comprises a first buffer and a second buffer, said control well comprises a sample buffer, and each of said testing wells and control well comprises a protein sample;
- b) a first testing well series of said plurality of testing wells, wherein each well of said first testing well series comprises a different ethylene glycol concentration;
- c) a second testing well series of said plurality of testing wells, wherein each well of said second testing well series comprises a different polyethylene glycol 3350 MME concentration;
- d) a third testing well series of said plurality of testing wells, wherein each well of said third testing well series comprises a different erythritol concentration;
- e) a fourth testing well series of said plurality of testing wells, wherein each well of said fourth testing well series comprises a different 2-methyl-2,4 pentanediol concentration;
- f) a fifth testing well series of said plurality of testing wells, wherein each well of said fifth testing well series comprises a different polyethylene glycol 6000 concentration;
- g) a sixth testing well series of said plurality of testing wells, wherein each well of said sixth testing well series comprises a different sodium malonate concentration;
- h) a seventh testing well series of said plurality of testing wells, wherein each well of said seventh testing well series comprises a different ethanol concentration;
- i) an eighth testing well series of said plurality of testing wells, wherein each well of said eighth testing well series comprises a different polyethylene glycol 10,000 concentration;
- j) a ninth testing well series of said plurality of testing wells, wherein each well of said ninth testing well series comprises an different glycerol concentration;
- k) a tenth testing well series of said plurality of testing wells, wherein each well of said tenth testing well series comprises a different xylitol concentration;
- l) an eleventh testing well series of said plurality of testing wells, wherein each well of said eleventh testing well series comprises a different sucrose concentration;
- m) a twelfth testing well series of said plurality of testing wells, wherein each well of said twelfth testing well series comprises a different trehalose dihydrate concentration;
- n) a thirteenth testing well series of said plurality of testing wells, wherein each well of said thirteenth testing well series comprises a different 1,6 hexanediol concentration;
- and
- n) a fourteenth testing well series of said plurality of testing wells, wherein each well of said fourteenth testing well series comprises a different 1,2 propanediol concentration.
13 more elements
A device, comprising:
- a) a solid substrate comprising a plurality of testing wells and a control well, wherein each of said plurality of testing wells comprises a first buffer and a second buffer, said control well comprises a sample buffer, and each of said testing wells and control well comprises a protein sample;
- b) a first testing well series of said plurality of testing wells comprising a first hydrogen ion concentration, wherein each well of said first testing well series comprises a different sodium chloride concentration;
- c) a second testing well series of said plurality of testing wells comprising a second hydrogen ion concentration, wherein each well of said second testing well series comprises a different sodium chloride concentration;
- d) a third testing well series of said plurality of testing wells comprising a third hydrogen ion concentration, wherein each well of said third testing well series comprises a different sodium chloride concentration;
- e) a fourth testing well series of said plurality of testing wells comprising a fourth hydrogen ion concentration, wherein each well of said fourth testing well series comprises a different sodium chloride concentration;
- f) a fifth testing well series of said plurality of testing wells comprising a fifth hydrogen ion concentration, wherein each well of said fifth testing well series comprises a different sodium chloride concentration;
- g) a sixth testing well series of said plurality of testing wells comprising a sixth hydrogen ion concentration, wherein each well of said sixth testing well series comprises a different sodium chloride concentration;
- h) a seventh testing well series of said plurality of testing wells comprising a seventh hydrogen ion concentration, wherein each well of said seventh testing well series comprises a different sodium chloride concentration;
- i) an eighth testing well series of said plurality of testing wells comprising an eighth hydrogen ion concentration, wherein each well of said eighth testing well series comprises a different sodium chloride concentration;
- and
- j) a ninth testing well series of said plurality of testing wells comprising a ninth hydrogen ion concentration, wherein each well of said ninth testing well series comprises a different sodium chloride concentration.
8 more elements
Dependencies are inferred from the claim text.