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US 11,001,824 B1Granted
Carbohydrate-binding protein
AssigneeUniversity of Georgia Research Foundation, Inc.
InventorsKausar N. Samli, Robert J. Woods, Loretta Yang
Life of this patent
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Abstract
The carbohydrate processing enzyme PNGase F was catalytically inactivated through mutation. Additional mutations yielded a catalytically inactive carbohydrate-binding protein with lectin-like properties including high affinity and specificity N-linked glycans, O-linked glycans, or both.
Claims23
A catalytically inactive carbohydrate-binding PNGase F protein, said protein having a plurality of amino acid mutations compared to a corresponding wild-type PNGase F protein, said plurality of mutations comprising
- (a) at least one first mutation that reduces or eliminates the catalytic activity of the PNGase F protein;
- and
- (b) at least one second mutation that affects binding affinity or binding specificity;
- wherein the second mutation comprises a mutation that enhances binding affinity to an N-linked glycan;
- and
- wherein at least one second mutation comprises a mutation at amino acid position S154 and/or R248 in E. meningosepticum PNGase F (SEQ ID NO:1), or a corresponding position in a homologous PNGase F sequence.
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Dependencies are inferred from the claim text.