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US 11,001,813 B2Granted
Method for purificating and crystallizating LPOR protein and use thereof
AssigneeZhejiang Shance HeQiShi Bio-Sci&Tech.Co., Ltd.
InventorsQi Cheng, Wenli Sun
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Abstract
Provided are a method for purifying and crystallizing the LPOR protein, wherein a protein and a crystal with complete structure can be obtained. Also provided are the three-dimensional spatial structure and binding site of the LPOR protein, and the use of the protein.
Claims16
A method for protein crystallization of a light-dependent protochlorophyllide oxidoreductase (LPOR), the method comprising:
- providing a purified and concentrated LPOR protein sample, and crystallizing the sample in a crystallization buffer at pH 4.5 to obtain a crystal protein, wherein the crystallization buffer comprises 18% PEG20000 and 0.1M sodium acetate.
Dependencies are inferred from the claim text.