Nicotiana nucleic acid molecules and uses thereof
AssigneeU.S. Smokeless Tobacco Company LLC
InventorsDongmei Xu, Mark T. Nielsen, Yanxin Shen
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Abstract
The present invention features Nicotiana nucleic acid sequences such as sequences encoding constitutive, or ethylene or senescence induced polypeptides, in particular cytochrome p450 enzymes, in Nicotiana plants and methods for using these nucleic acid sequences and plants to alter desirable traits, for example by using breeding protocols.
Claims23
A Nicotiana plant, or part thereof, comprising a recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a nucleotide sequence encoding a nicotine demethylase sequence having at least 95% sequence identity to SEQ ID NO:
- 3.
A method comprising the steps of:
- a. obtaining a Nicotiana plant comprising a recombinant nucleic acid molecule comprising a nucleotide sequence encoding a nicotine demethylase sequence having at least 95% amino acid sequence identity to SEQ ID NO:
- 3;
- b. crossing said Nicotiana plant to a second Nicotiana plant lacking said recombinant nucleic acid molecule;
- and
- c. producing at least one progeny Nicotiana plant from said crossing wherein said progeny Nicotiana plants comprise a nucleotide sequence encoding the nicotine demethylase sequence set forth in SEQ ID NO:
- 3.
A Nicotiana plant, or part thereof, comprising a genomic modification in an endogenous gene encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:
- 3, wherein said genomic modification confers increased expression of said endogenous gene.
Dependencies are inferred from the claim text.