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US 10,995,326 B2Granted
Method for producing substrate culture product and substrate culture product
AssigneeFujiwara Techno-Art Co., Ltd.
InventorsMasahiro Kariyama, Akira Mori, Satoko Takahashi
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Abstract
Provided is a method for safely and selectively producing a substrate culture product including a large amount of a desired degrading enzyme. A method for producing a substrate culture product used for feedstuff includes inoculating filamentous fungi bred so that a target degrading enzyme is produced by self-cloning in high productivity on a substrate, and producing the substrate culture product having functionality by ventilating the substrate to carry out solid culture.
Claims12
A method for producing a culture product used for feedstuff, the method comprising:
- inoculating a filamentous fungi on a solid substrate,
- wherein said filamentous fungi expresses an increased amount of an exogenous target degrading enzyme compared to a non-modified filamentous fungi, and
- wherein said exogenous target enzyme is a target enzyme from the same species of said filamentous fungi;
- culturing said filamentous fungi on said solid substrate;
- and
- ventilating by supplying external air to produce the culture product;
- wherein said culture product is in a solid-form which includes the target degrading enzyme.
4 more elements
Dependencies are inferred from the claim text.