Processes to prepare elongated 2-ketoacids and C5-C10 compounds therefrom via genetic modifications to microbial metabolic pathways
AssigneeDow Global Technologies LLC
InventorsParesh C. Sanghani, Sarah Delaplane
Life of this patent
Estimating the term…
Abstract
Genetically modified isopropylmalate synthases, processes for preparing a C7-C11 2-ketoacids utilizing genetically modified isopropylmalate synthases, and microbial organisms including genetically modified isopropylmalate synthases are described. The genetically modified isopropylmalate synthases, processes for preparing a C7-C11 2-ketoacids, and microbial organisms including genetically modified isopropylmalate synthases can be particularly useful for producing corresponding Cn_1 aldehydes, alcohols, carboxylic acids, and Cn_2 alkanes both in vivo and in vitro.
Claims7
A process for preparing a C7-C11 2-ketoacid, the process comprising:
- (I) providing at least one of a C4-C10 2-ketoacid substrate, with:
- (a) a genetically modified isopropylmalate synthase (IPMS) comprising at least one of:
- (i) an isopropylmalate synthase variant comprising the amino acid sequence variant of SEQ ID NO:
- 1 having mutations H97A, S139G, N167G, P169A, G181A, A182G, G210A, A214S, and G462D in the SEQ ID NO:
- 1 and having IPMS activity;
- (ii) isopropylmalate synthase variant comprising the amino acid sequence variant comprising SEQ ID NO:
- 2 having the mutations H97A, S139G, N167G, P169A, M255L, R260A, N264Q, and G462D in the SEQ ID NO:
- 2 and having IPMS activity;
- or (iii) isopropylmalate synthase variant comprising the amino acid sequence variant of comprising SEQ ID NO:
- 2 having the mutations H97A, S139G, N167G, P169A, D348E, D350E, M353L, Q355N, and G462D in the SEQ ID NO:
- 2 and having IPMS activity
- (b) a isopropylmalate isomerase having isopropylmalate isomerase activity;
- and
- (c) a isopropylmalate dehydrogenase having isopropylmalate dehydrogenase activity;
- under conditions that the at least one of the C4-C10 2-ketoacid substrate is converted to the C7-C11 2-ketoacid;
- and wherein the conversion of the at least one of the C4-C10 2-ketoacid substrate to the C7-C11 2-ketoacid occurs via one or more biochemical reactions.
A genetically modified isopropylmalate synthase (IPMS) polypeptide having isopropylmalate synthase activity, the polypeptide comprising at least one of:
- (a) an isopropylmalate synthase variant comprising the amino acid sequence variant of SEQ ID NO:
- 1 having mutations H97A, S139G, N167G, P169A, G181A, A182G, G210A, A214S, and G462D in the SEQ ID NO:
- 1 and having IPMS activity;
- (b) isopropylmalate synthase variant comprising the amino acid sequence variant comprising SEQ ID NO:
- 2 having the mutations H97A, S139G, N167G, P169A, M255L, R260A, N264Q, and G462D in the SEQ ID NO:
- 2 and having IPMS activity;
- or (c) isopropylmalate synthase variant comprising the amino acid sequence variant of comprising SEQ ID NO:
- 2 having the mutations H97A, S139G, N167G, P169A, D348E, D350E, M353L, Q355N, and G462D in the SEQ ID NO:
- 2 and having IPMS activity.
Dependencies are inferred from the claim text.