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US 10,995,114 B2Granted
Scalable production of genetically engineered nanofibrous macroscopic materials via filtration
AssigneePresident and Fellows of Harvard College
InventorsNeel Satish Joshi, Noémie-Manuelle Dorval Courchesne, Anna M. Duraj-Thatte, Peter Q. Nguyen
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Abstract
Provided herein are filtration-based purification methods for amyloid fibers, such as curli fibers, directly from microbial culture, and their fabrication into free-standing thin films. Additionally, methods for recycling amyloid fibers thing films by, for example, disassembly and re-assembly, are disclosed herein.
Claims11
A method of producing an amyloid fiber thin film comprising:
- contacting a composition comprising amyloid fibers with a filter membrane;
- washing the filter membrane;
- crosslinking the amyloid fibers on the filter membrane using a crosslinking agent, thereby producing crosslinked amyloid fibers;
- placing a second membrane on top of the crosslinked amyloid fibers, such that the crosslinked amyloid fibers are positioned between the filter membrane and the second membrane, wherein the second membrane is of a different material than the filter membrane;
- dissolving the filter membrane with a solvent;
- drying the crosslinked amyloid fibers on the second membrane;
- and
- removing the crosslinked amyloid fibers from the second membrane;
- thereby producing a amyloid fiber thin film.
6 more elements
Dependencies are inferred from the claim text.