Glucosyltransferase enzymes for production of glucan polymers
AssigneeNutrition & Biosciences USA 4, Inc.
InventorsMark S. Payne, Yefim Brun, Hongxian He, Thomas Scholz
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Abstract
Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.
Claims22
A composition comprising (i) an insoluble poly alpha-1,3-glucan having at least 90% alpha-1,3 glycosidic linkages, and (ii) a genetically engineered cell that expresses an isolated glucosyltransferase enzyme comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:4.
Dependencies are inferred from the claim text.