Method for producing a bioproduct selected from acetone, isopropanol and combinations thereof
AssigneeWhite Dog Labs, Inc.
InventorsBryan Patrick Tracy, Shawn William Jones, Daniel Knox Mitchell, Aharon M. Eyal, Karthikeyan Ramachandriya
Life of this patent
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Abstract
Methods for producing a bioproduct selected from acetone, isopropanol and a combination thereof with a microorganism in a fermentor are disclosed. The methods include separating cells of the microorganism from a fermentation broth to form separated cells and recycling at least a fraction of the separated cells to the fermentor to achieve one or more of the following: (1) cell concentration in said fermentor greater than 2 g/L; mass yield on a first feedstock greater than 32%; productivity greater than 0.12 g/L/h; and bioproduct titer greater than 10 g/L.
Claims18
A method for producing a bioproduct selected from acetone, isopropanol and a combination thereof, comprising
- (i) providing a first feedstock, a nitrogen source and optionally a second feedstock to form a fermentation medium;
- (ii) providing an isolated organism capable of metabolizing CO2 into acetyl-CoA;
- (iii) culturing said organism in a fermenter in said fermentation medium whereby said first feedstock and optionally said second feedstock are metabolized and a fermentation broth is formed, which broth comprises said bioproduct;
- (iv) separating cells from said fermentation broth to form separated cells;
- (v) recycling at least a fraction of said separated cells to the fermenter;
- and
- (vi) separating said bioproduct to form separated bioproduct;
- which producing is characterized by one or more of the following criteria
- a. cell concentration in said fermenter being greater than 2 g/L;
- b. mass yield on first feedstock being greater than 32%;
- c. productivity being greater than 0.12 g/L/h;
- and
- d. total bioproduct titer being greater than 10 g/L.
Dependencies are inferred from the claim text.