Methods and materials for producing 7-carbon monomers
AssigneeINV Nylon Chemicals Americas, LLC
InventorsAlex Van Eck Conradie, Adriana Leonora Botes, Jonathan Kennedy, Nadia Fatma Kadi
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Abstract
This document describes biochemical pathways for producing 7-aminoheptanoic acid using a β-ketoacyl synthase or a β-ketothiolase to form either a 5-amino-3-oxopentanoyl-[ACP] or 5-amino-3-oxopentanoyl-CoA intermediate. 7-aminoheptanoic acid can be enzymatically converted to pimelic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol or the corresponding salts thereof. This document also describes recombinant microorganisms producing 7-aminoheptanoic acid as well as pimelic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol or the corresponding salts thereof.
Claims31
A method of producing 5-amino-3-oxopentanoyl-[ACP] or 5-amino-3-oxopentanoyl-CoA or corresponding salts thereof, said method comprising enzymatically converting β-alanyl-[ACP] or β-alanyl-CoA respectively to 5-amino-3-oxopentanoyl-[ACP] or 5-amino-3-oxopentanoyl-CoA or corresponding salts thereof, using a polypeptide having the activity of a β-ketoacyl synthase or a β-ketothiolase classified under EC. 2.3.1.- and/or a CoA transferase classified under EC 2.8.3-.
A method for biosynthesizing 7-aminoheptanoate, said method comprising enzymatically synthesizing 5-amino-3-oxopentanoyl-[ACP] or 5-amino-3-oxopentanoyl-CoA or the salts thereof from β-alanyl-[ACP] or β-alanyl-CoA using a polypeptide having the activity of a β-ketoacyl synthase or a β-ketothiolase classified under EC. 2.3.1.- and/or a CoA transferase classified under EC 2.8.3-, and enzymatically converting 5-amino-3-oxopentanoyl-[ACP] or 5-amino-3-oxopentanoyl-CoA or the salts thereof to 7-aminoheptanoate.
A non-naturally occurring biochemical network comprising β-alanyl-[ACP] or β-alanyl-CoA, an exogenous nucleic acid encoding a polypeptide having the activity of a β-ketothiolase or a β-ketoacyl synthase classified under EC. 2.3.1, and a 5-amino-3-oxopentanoyl-[ACP] or 5-amino-3-oxopentanoyl-CoA respectively.
A non-naturally occurring biochemical network comprising at least one exogenous nucleic acid encoding a polypeptide having the enzymatic activity of (i) a β-ketoacyl synthase and/or a β-ketothiolase, (ii) a thioesterase or a CoA transferase, and one or more of (iii) a 3-hydroxyacyl-CoA dehydrogenase or a 3-oxoacyl-[ACP] reductase, (iv) an enoyl-CoA hydratase or 3-hydroxyacyl-[ACP] dehydratase, and (v) a trans-2-enoyl-CoA reductase or enoyl-[ACP] reductase, said microorganism producing 7-aminoheptanoate or the corresponding salt thereof.
A method of producing 5-amino-3-oxopentanoyl-CoA or a salt thereof, said method comprising enzymatically converting β-alanine to 5-amino-3-oxopentanoyl-CoA or a salt thereof using a polypeptide having the activity of a β-ketoacyl synthase or a β-ketothiolase classified under EC. 2.3.1.-, further comprising enzymatically converting β-alanine or the salt thereof to 5-amino-3-oxopentanoyl-CoA using polypeptides having the enzymatic activities of a β-ketothiolase, CoA transferase, acetyl-CoA hydrolase, succinyl-CoA:
- acetate CoA-transferase, and thiolase.
Dependencies are inferred from the claim text.