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AssigneeQinhuangdao Huaheng Bioengineering Co., Ltd.
InventorsBian Wu, Yang Liu, Ruifeng Li, Dongzhu Zhang, Zhicheng Liu, Hui Zhang
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The present invention discloses an aspartase variant, method of preparing the same and use thereof. Compared with sequence 2 in the sequence listing, the amino acid sequence of the aspartase variant provided in the present invention has more than 96% identity, and there are mutations in T187I and N326C at positions 187 and 326, respectively, and has improved catalytic activity for the ammoniation of acrylic acid, compared with the aspartase shown in sequence 2. The experiment proves that the aspartase variant provided in the present invention does have improved catalytic activity for the ammoniation of acrylic acid compared with the wild type parent aspartase, and has better thermal stability and pH spectrum. The reaction can greatly increase the conversion ratio of acrylic acid.
An aspartase variant, the amino acid sequence thereof is more than 96% identical to SEQ ID NO. 2 in the sequence listing, and has mutations of T187I and N326C at both positions 187 and 326 of SEQ ID NO. 2, and the aspartase variant has improved catalytic activity for the ammoniation of acrylic acid compared with that of aspartase shown in SEQ ID NO. 2.
Dependencies are inferred from the claim text.