Back to results
US 10,988,740 B2Granted
Development of microorganisms for hydrogen production
AssigneeSvetlana Oard
InventorSvetlana Oard
Life of this patent
Estimating the term…
Abstract
Methods and compositions are provided for engineering microorganisms, which permit enhanced H2 production. The methods and compositions provided include novel chimeric gene constructs encoding H2-forming H2ase and maturation proteins, allowing for generation of H2 continuously in large quantities. In one illustrated embodiment, novel engineered algae are provided with increased levels of H2 production.
Claims9
A microorganism, comprising a modified gene encoding a H2-forming H2ase and one or more genes encoding maturation proteins that mediate the maturation of said H2ase;
- wherein a nucleic acid molecule encoding said H2ase is genetically engineered for high expression at more than 2% O2 by silent substitutions of nucleotides, wherein said H2ase gene is expressed at an O2 concentration of about 0% to more than 2%;
- wherein at least one of said genes encoding a maturation protein is expressed under less than 0.1% O2;
- wherein said microorganism is selected from the group consisting of algae, bacteria, and cyanobacteria;
- exhibiting an increased level of H2 production when compared to an otherwise identical microorganism, but lacking said H2ase gene and the engineered genes encoding maturation proteins.
1 more element
Dependencies are inferred from the claim text.