Elastomeric proteins
AssigneeBolt Threads, Inc.
InventorsJoshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
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Abstract
This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.
Claims53
A method for producing a composition comprising a recombinant resilin protein, comprising:
- culturing a population of recombinant host cells in a fermentation, wherein said recombinant host cells comprise a vector comprising a secreted resilin coding sequence, and wherein said recombinant host cells secrete a recombinant resilin protein encoded by said secreted resilin coding sequence, and wherein said recombinant host cells are yeast cells;
- and
- purifying said recombinant resilin protein from said fermentation.
A vector comprising more than one secreted resilin coding sequence.
A method for producing a composition comprising a recombinant resilin protein, comprising:
- culturing a population of recombinant host cells in a fermentation, wherein said recombinant host cells comprise a vector comprising a secreted resilin coding sequence, wherein said recombinant host cells secrete a recombinant resilin protein encoded by said secreted resilin coding sequence, and wherein said recombinant host cells produce the recombinant resilin at a rate of greater than 2 mg resilin/g dry cell weight/hour;
- and
- purifying said recombinant resilin protein from said fermentation.
A method for producing a composition comprising a recombinant resilin protein, comprising:
- culturing a population of recombinant host cells in a fermentation, wherein said recombinant host cells comprise a vector comprising more than one secreted resilin coding sequence, and wherein said recombinant host cells secrete a recombinant resilin protein encoded by said secreted resilin coding sequence;
- and
- purifying said recombinant resilin protein from said fermentation.
Dependencies are inferred from the claim text.