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US 10,987,648 B2Granted
Functionalized surfaces and preparation thereof
AssigneeTwist Bioscience Corporation
InventorsBill James Peck, Pierre Indermuhle, Eugene P. Marsh, Andres Fernandez, David Stern
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Abstract
Compositions, devices, methods and systems are provided for differential functionalization of a surface of a structure to support biopolymer synthesis. Provided herein are processes which include use of lamps, lasers, and/or microcontact printing to add functional groups to surfaces for the efficient and uniform synthesis of oligonucleic acids.
Claims20
A method for surface patterning, the method comprising:
- applying a first set of molecules to a surface of a structure, wherein each of the first set of molecules binds to the surface and comprises a reactive group capable of binding to a nucleoside;
- synthesizing a first layer of oligonucleotides, wherein each oligonucleotide in the first layer of oligonucleotides comprises about 10 to about 100 bases in length and extends from the surface;
- applying electromagnetic radiation (EMR) to a predetermined region of the surface to selectively remove a portion of the layer of oligonucleotides, wherein the EMR comprises a wavelength from about 100 nm to about 300 nm;
- and
- synthesizing a second layer of oligonucleotides, wherein each oligonucleotide in the second layer of oligonucleotides extends oligonucleotides from the remaining portion of the first layer of layer of oligonucleotides.
2 more elements
Dependencies are inferred from the claim text.