Diagnostic methods for the detection and quantification of blood-related diseases
AssigneeAdolphe Merkle Institute, University of Fribourg
InventorsNico Bruns, Omar Rifaie Graham, Jonas Pollard
Life of this patent
Estimating the term…
Abstract
A diagnostic method suitable for detection and quantification of blood-related diseases or conditions. The methods utilize biomarkers, such as hemoglobin and hemozoin as catalysts in an atom transfer radical polymerization (ATRP) reaction performed above a lower critical solution temperature (LCST) of a polymer which allows the polymerization to be tracked by rate of turbidity formation. The rate of turbidity formation is correlated to the concentration of the biomarker, making the tests useful quantitative techniques which can be utilized as point-of-care tests in the field.
Claims15
A diagnostic method for the detection of a biomarker in a biological sample comprising the steps of:
- performing a polymerization reaction above a lower critical solution temperature (LCST) of a polymer formed by combining a plurality of initiators, a plurality of atom transfer radically polymerizable monomers, and a biological sample containing a biomarker that serves as a catalyst for atom transfer radical polymerization reaction (ATRP), whereby the polymer formed by the monomers is precipitable at or above the lower critical solution temperature (LCST);
- determining turbidity formation;
- and
- determining a concentration of the biomarker in the biological sample based on the turbidity formation.
A diagnostic method for the detection of a biomarker in a biological sample comprising the steps of:
- A) performing a polymerization reaction above a lower critical solution temperature (LCST) of a polymer formed by combining a plurality of initiators, a plurality of atom transfer radically polymerizable monomers, and a biological sample containing a biomarker that serves as a catalyst for an atom transfer radical polymerization reaction (ATRP), whereby the polymer formed by the monomers is precipitable at or above the lower critical solution temperature (LCST);
- B) determining turbidity at i) a point in time after initiation of polymerization or ii) a time to reach a certain turbidity after the initiation, or both i) and ii), and
- C) determining a concentration of the biomarker in the biological sample based on the determination made in step B.
Dependencies are inferred from the claim text.