Method for producing meso-galactaric acid by contacting a fungal cell with a biomaterial having galacturonic acid
AssigneeTeknologian tutkimuskeskus VTT Oy
InventorsJoosu Kuivanen, Peter Richard, Ying-Mong Jasmin Wang
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Abstract
The present invention relates to a field of genetically modified fungal cells and converting galacturonic acid to meso-galactaric acid, more precisely to a method of producing meso-galactaric acid. The invention further relates to recombinant fungal cells having a specific combination of modifications including but not limited to expression of uronate dehydrogenase enzyme, reduced D-galacturonic acid reductase activity, and furthermore reduced meso-galactaric acid catabolism, as well as uses and methods related thereto.
Claims12
A method for producing meso-galactaric acid, said method comprising
- contacting a fungal cell genetically modified to express uronate dehydrogenase enzyme, genetically modified to have reduced D-galacturonic acid reductase activity, and modified to reduce meso-galactaric acid catabolism by deleting at least part of a gene encoding JGI protein IDs:
- 39114 (SEQ ID No:
- 72), 1090836 (SEQ ID NO:
- 73) and/or 1121140 (SEQ ID NO:
- 74), with a biomaterial comprising galacturonic acid, and
- recovering the resulting meso-galactaric acid.
A fungal cell that has been genetically modified to express uronate dehydrogenase enzyme, genetically modified to have reduced D-galacturonic acid reductase activity, and is capable of converting D-galacturonic acid to meso-galactaric acid, wherein said fungal cell has been further modified to reduce meso-galactaric acid catabolism by deleting at least part of a gene encoding JGI protein IDs:
- 39114 (SEQ ID No:
- 72), 1090836 (SEQ ID NO:
- 73) and/or 1121140 (SEQ ID NO:
- 74).
A method for treating biomaterial comprising galacturonic acid, which method comprises that a fungal cell genetically modified to express uronate dehydrogenase enzyme, genetically modified to have reduced D-galacturonic acid reductase activity, and modified to reduce mesogalactaric acid catabolism by deleting at least part of a gene encoding JGI protein IDs:
- 39114 (SEQ ID No:
- 72), 1090836 (SEQ ID NO:
- 73) and/or 1121140 (SEQ ID NO:
- 74), is contacted with said biomaterial under suitable culture conditions.
Dependencies are inferred from the claim text.