Escherichia coli transformant for producing itaconate and uses thereof

An Escherichia coli transformant, comprising at least one plasmid, wherein the at least one plasmid includes a D-xylose dehydrogenase (XDH) gene, a D-xylonolactonase (XL) gene, a 2-keto-3-deoxy-D-xylonate dehydratase (KdxD) gene, and a 2-ketoglutarate semialdehyde dehydrogenase (KGSADH) gene;

• wherein an isocitrate dehydrogenase (icd) gene of the Escherichia coli transformant is deleted;
• wherein the at least one plasmid further includes a D-xylonate dehydratase (XD) gene;
• wherein the XDH gene, the XL gene, the KdxD gene, the KGSADH gene, and the XD gene are obtained from Burkholderia xenovorans strains;
• wherein the XDH gene, the XL gene, the XD gene, the KdxD gene, and the KGSADH gene are sequentially constructed from their 5′ ends to 3′ ends in the at least one plasmid;
• wherein the Escherichia coli transformant is a transformant of Escherichia coli BW25113;
• wherein the Escherichia coli transformant converts xylose into α-ketoglutaric acid through a nonphosphorylative metabolic pathway;
• wherein a xylose isomerase gene (xylA), a 2-dehydro-3-deoxy-D-pentonate aldolase gene (yjhH), and a 2-keto-3-deoxygluconate aldolase gene (yagE) of the Escherichia coli transformant are deleted;
• wherein the Escherichia coli transformant produces itaconate without adding a yeast extract or glutamate;
• and
• wherein the at least one plasmid is pKL7 and pKL8.