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US 10,982,236 B2Granted
Recombinant yeast for producing 2,3-butanediol including pyruvate decarboxylase derived from candida tropicolis and method for producing 2,3-butanediol using the same
AssigneeSeoul National University R&Db Foundation
InventorsJin Ho Seo, Jin Woo Kim
Life of this patent
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Abstract
Provided is a recombinant Saccharomyces cerevisiae for producing 2,3-butanediol, wherein all GPD1 and GPD2 genes involved in glycerol biosynthesis are removed and a gene encoding NADH oxidase is introduced, and wherein pyruvate decarboxylase activity is inactivated and Candida tropicalis PDC1 gene encoding Candida tropicalis pyruvate decarboxylase 1-is introduced, and wherein expression of the Candida tropicalis PDC1 gene is regulated by a GPD2 promoter.
Claims9
A recombinant Saccharomyces cerevisiae for producing 2,3-butanediol,
- wherein GPD1 and GPD2 genes encoding glycerol-3-phosphate dehydrogenase are removed and a gene encoding an NADH oxidase is introduced in the recombinant Saccharomyces cerevisiae,
- wherein a pyruvate decarboxylase gene is inactivated and a Candida tropicalis PDC1 gene encoding Candida tropicalis pyruvate decarboxylase 1 is introduced in the recombinant Saccharomyces cerevisiae, wherein the Candida tropicalis PDC1 gene is operably linked to a GPD2 promoter, and
- wherein the recombinant Saccharomyces cerevisiae is transformed so as to express acetolactate synthase, is transformed so as to express acetolactate decarboxylase, and is transformed so as to express butanediol dehydrogenase.
Dependencies are inferred from the claim text.