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US 10,982,232 B2Granted
Diterpene synthases and their use for production of diterpenes
AssigneeThe Regents of the University of California
InventorsPhilipp Zerbe, Sibongile Mafu
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Abstract
Diterpene synthases and methods of their use are described herein.
Claims20
An isolated nucleic acid molecule, comprising a promoter operably linked to a sequence of nucleotides encoding a diterpene synthase polypeptide at least 95% identical to SEQ ID NO:1 or an active fragment thereof wherein the diterpene synthase polypeptide catalyzes the formation of sibongilene from geranylgeranyl diphosphate (GGPP), and wherein the promoter is not native to the sequence of nucleotides.
A host cell comprising nucleic acid molecule;
- comprising a sequence of nucleotides encoding a diterpene synthase polypeptide at least 95% identical to SEQ ID NO:1 or an active fragment thereof, wherein the diterpene synthase polypeptide catalyzes the formation of sibongilene from geranylgeranyl diphosphate (GGPP), wherein the encoded diterpene synthase polypeptide is heterologous to the host cell.
Dependencies are inferred from the claim text.