Protein arrays and methods of using and making the same
AssigneeGen9, Inc.
InventorsJoseph Jacobson, Michael E. Hudson, Larry Li-Yang Chu
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Abstract
Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids.
Claims10
A method for preparing a plurality of proteins comprising:
- (a) providing a plate comprising a plurality of wells,
- wherein the plurality of wells comprises a plurality of nucleic acids, each nucleic acid having a predefined sequence and encoding a protein, and
- wherein the nucleic acids of each well are immobilized on one or more supports;
- and
- (b) expressing in vitro a plurality of proteins from the plurality of nucleic acids,
- wherein the proteins are expressed in the wells of the plate in volumes having a size of 0.5 picoliters to 100 nanoliters.
Dependencies are inferred from the claim text.