Beta-lactamase variants
AssigneesDa Volterra, Bioaster
InventorsJean De Gunzburg, Jean-Denis Docquier
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Abstract
The present invention relates to an isolated polypeptide having beta-lactamase activity and nucleic acid sequences encoding the polypeptide. The isolated polypeptide of the invention is a Verona integron-encoded metallo-β-lactamase (VIM-2) variant with improved properties such as improved protease stability, stability in intestinal medium, improved activity against one or more antibiotics, improved specific activity and/or improved production in a host cell.
Claims20
An isolated polypeptide having beta-lactamase activity, which comprises an amino acid sequence having beta-lactamase activity, wherein said amino acid sequence has at least 90% sequence identity to SEQ ID NO:1, said amino acid sequence comprising substitutions in position 34 and in at least one position selected from positions 22 and 130, wherein the positions correspond to the positions in SEQ ID NO:1.
Dependencies are inferred from the claim text.