Engineered mammalian cells for production of recombinant proteins
AssigneesDanmarks Tekniske Universitet, The Regents of the University of California
InventorsBjørn Gunnar Voldborg, Lasse Ebdrup Pedersen, Bernhard Palsson
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Abstract
The present invention relates to mammalian cells modified to provide for improved expression of a recombinant protein of interest. In particular, the invention relates to CHO cells and other host cells in which the expression of one or more endogenous secreted proteins has been disrupted, as well as to the preparation, identification and use of such cells in the production of recombinant proteins.
Claims12
A Chinese hamster ovary (CHO) cell modified to disrupt expression of one or more endogenous proteins selected from the group consisting of Tissue inhibitors of metalloproteases type 1 (TIMP1), Nidogen 1 isoform 1 (NID1-1), Nidogen 1 isoform 2 (NID1-2), Biglycan (BGN), and Lectin, galactoside-binding soluble 3 binding protein (LGALS3BP).
Dependencies are inferred from the claim text.