US 10,982,015 B2 · PEG-ACS/M-siRNA nanocomposite, application thereof, and method for reducing histamine content during fishmeal storage

Abstract

The present invention provides a PEG-ACS/M-siRNA nanocomposite application, and method for reducing the histamine content during fishmeal storage thereof. A small interfering ribonucleic acid (siRNA) is designed and prepared according to a histidine decarboxylase gene of Morganella morganii (Morganella morganii subsp. morganii KT), and the histidine decarboxylase gene has a sequence of SEQ ID No: 1. A PEG-ACS/M-siRNA nanocomposite is prepared by using a PEGylated arginine-modified chitosan as a carrier. A PEG-ACS/M-siRNA nanocomposite is added to fishmeal in a certain ratio. The method for reducing the histamine content during fishmeal storage has a significant inhibitory effect on the histamine content during fishmeal storage, and can reduce the histamine content in fishmeal by 49%-53%, which has great significance for the control of biogenic amines in fishmeal in the feed industry.

Claims20

Dependent claims

A PEGylated arginine-modified chitosan/small-interfering RNA (PEG-ACS/siRNA) nanocomposite, comprising a small-interfering RNA (siRNA), wherein the siRNA is designed according to a histidine decarboxylase gene of Morganella morganii (Morganella morganii subsp. morganii KT);

and the histidine decarboxylase gene has the sequence of SEQ ID No:
1;
and the PEG-ACS/siRNA nanocomposite is prepared by using a PEGylated arginine-modified chitosan (PEG-ACS) as a carrier.

A method for reducing a histamine content in fishmeal storage, wherein a PEG-ACS/small-interfering RNA (siRNA) nanocomposite is added to the fishmeal storage, wherein a PEG-ACS/siRNA nanocomposite is added to the fishmeal, at an addition ratio of 3-6 g of the PEG-ACS/siRNA nanocomposite per kilogram of the fishmeal;

wherein the PEG-ACS/siRNA nanocomposite nanocomposite comprises a siRNA;
and wherein the siRNA is designed according to a histidine decarboxylase gene of Morganella morganii (Morganella morganii subsp. morganii KT);
and the histidine decarboxylase gene has the sequence of SEQ ID No:
1;
and the PEG-ACS/siRNA nanocomposite is prepared by using a PEGylated arginine-modified chitosan as a carrier.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/133,716Filed 18 Sep 2018

Priority

CN 201810739772.56 Jul 2018

Published

US 2020/0010574 A19 Jan 2020

Granted

20 Apr 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification13 codes

C08B 37/003
2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof: chitin, i.e. 2-acetamido-2-deoxy--D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan,…
C07H 21/02
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of…: with…
C08L 5/08
Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00: chitin; Chondroitin sulfate; Hyaluronic…
C12N 15/52
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: genes encoding for enzymes or proenzymes

PEG-ACS/M-siRNA nanocomposite, application thereof, and method for reducing histamine content during fishmeal storage

Life of this patent

PEG-ACS/M-siRNA nanocomposite, application thereof, and method for reducing histamine content during fishmeal storage

Life of this patent

Abstract

Claims20