Compositions and methods for fabricating synthetic dragline spider silk
AssigneeSeevix Material Sciences Ltd.
InventorsShmulik Ittah, Meni Shimel, Uri Gat
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Abstract
Compositions comprising a mixture of proteins derived from MaSP, nucleic acids encoding same and method for the preparation of synthetic dragline spider silk are provided. The compositions of the invention comprise a mixture of proteins of differing molecular weight, wherein each protein of said mixture comprises, independently, multiple repeats of a repetitive region of a MaSP (major ampullate spidroin) protein or a functional homolog, variant, derivative or fragment thereof.
Claims12
A composition comprising a mixture of proteins comprising between 4 and 32 types of proteins of differing molecular weight, wherein each protein in said mixture comprises, independently, 2 to 70 repeats of a repetitive region of an ADF-4 protein, comprising an amino acid sequence as set forth in SEQ ID NO:
- 1
- (X1)ZX2GPGGYGPX3X4X5GPX6GX7GGX8GPGGPGX9X10
- wherein X1 is, independently, at each instance A or G wherein at least 50% of (X1)Z is A, Z is an integer between 5 to 30;
- X2 is S or G;
- X3 is G or E;
- X4 is G, S or N;
- X5 is Q or Y;
- X6 is G or S;
- X7 is P or R;
- X8 is Y or Q;
- X9 is G or S;
- and X10 is S or G, wherein said mixture is characterized by molecular weight increment of 2 kDa to 3.5 kDa, between at least two proteins in said mixture.
An isolated nucleic acid sequence encoding 4 to 32 proteins of differing molecular weight,
- wherein each protein comprises, independently, 2 to 70 repeats of a repetitive region of an ADF-4 protein, comprising an amino acid sequence as set forth in SEQ ID NO:
- 1
- (X1)ZX2GPGGYGPX3X4X5GPX6GX7GGX8GPGGPGX9X10
- wherein X1 is, independently, at each instance A or G wherein at least 50% of (X1)Z is A, Z is an integer between 5 to 30;
- X2 is S or G;
- X3 is G or E;
- X4 is G, S or N;
- X5 is Q or Y;
- X6 is G or S;
- X7 is P or R;
- X8 is Y or Q;
- X9 is G or S;
- and X10 is S or G, wherein said proteins of differing molecular weight are characterized by molecular weight increment of 2 kDa to 3.5 kDa between at least two proteins.
Dependencies are inferred from the claim text.