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US 10,976,323 B2Granted
Methods for measuring concentrations of biomolecules in biofluids
AssigneeC2N Diagnostics
InventorsKristopher Michael Kirmess, Kevin Edward Yarasheski, Matthew R. Meyer
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Abstract
The present invention provides methods for measuring the absolute concentration of Tau, and other protein, peptide fragments and proteoforms in CSF and plasma samples collected from a subject. Such biomolecules may be implicated in one or more neurological and neurodegenerative diseases or disorders. Also provided is a method for determining whether a therapeutic agent affects the CSF or plasma concentration of a central nervous system derived biomolecule. Also provided are kits for performing the methods of the invention.
Claims8
A method comprising:
- a) isolating unlabeled Tau protein from a sample;
- b) obtaining unlabeled Tau protein fragments from the unlabeled Tau protein;
- and
- c) measuring the amount of at least two unlabeled Tau protein fragments by relating the ratio of a stable isotope-labeled Tau protein fragments quantitation internal standard to each of the at least two unlabeled Tau protein fragments, wherein:
- i) one of the at least two unlabeled Tau protein fragments is selected from the group consisting of SEQ ID NOs:
- 54, 74, 92 and 102;
- and
- ii) another of the at least two unlabeled Tau protein fragments is selected from the group consisting of SEQ ID NOs:
- 49-53, 55-91, 93-101 and 103-106.
6 more elements
Dependencies are inferred from the claim text.