Back to results
US 10,975,405 B2Granted
Method for do novo biosynthesis of chondroitin sulfate
AssigneeJiangnan University
InventorsZhen Kang, Jian Chen, Guocheng Du, Zhengxiong Zhou
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for chondroitin sulfate biosynthesis, belongs to the field of pharmaceuticals. CS was biosynthesized by sulfating the chondroitin with C4ST or C6ST in Tris-HCl buffer assisted with 3′-phosphoadenosine 5′-phophosulfate (PAPS). C4ST and C46ST came from bioengineered Escherichia coli or Pichia pastoris. Chondroitin came from bioengineered Bacillus subtilis 168.
Claims13
A method for biosynthesis of Chondroitin sulfate (CS), comprising:
- expressing in Escherichia coli or Pichia pastoris a heterogeneous gene encoding chondroitin 4-sulfotransferase (C4ST) and a heterogeneous gene encoding chondroitin 6-sulfotransferase (C6ST);
- wherein a sequence of the heterogeneous gene encoding C4ST is set forth in SEQ ID NO:15;
- and wherein a sequence of the heterogeneous gene encoding C6ST is set forth in SEQ ID NO:16;
- collecting the C4ST and C6ST;
- and incubating the C4ST and C6ST with a 3′-phosphoadenosine-5′-phosphosulfate (PAPS) regeneration system for 20-50 hours;
- and wherein the recombinant Escherichia coli or the Pichia pastoris comprise a recombinant expression plasmid comprising the heterogeneous gene encoding C4ST and the heterogeneous gene encoding C6ST.
3 more elements
Dependencies are inferred from the claim text.