PYL9 and uses thereof
AssigneePurdue Research Foundation
InventorsJian-Kang Zhu, Zhulong Chan, Ray Bressan, Yang Zhao
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Abstract
The present invention provides for transgenic plants and methods of producing such transgenic plants, wherein the transgenic plants express an increased amounts of PYL9 to interact with abscisic acid (ABA) thereby activating enhanced drought resistance and leaf senescence relative to control or wild-type plants.
Claims9
A transgenic plant having increased drought tolerance and leaf senescence transformed with polynucleotide sequence encoding SEQ ID NO:
- 104, which is a PYL9 protein, and operably linked to a polynucleotide sequence of a stress inducible rd29A promoter, wherein the transgenic plant exhibits increased expression of the PYL9 protein and interacts with abscisic acid (ABA) thereby activating enhanced drought resistance and senescence relative to a non-transformed plant.
A method of producing a transgenic plant having an increased tolerance to an environmental drought stress, said method comprising the steps of:
- (a) transforming plant cells with an DNA expression cassette comprising a DNA nucleotide sequence encoding an PYL9 protein having an amino acid sequence of SEQ ID NO. 104, and wherein the DNA nucleotide sequence encoding the PYL9 protein is operably linked to a nucleotide sequence encoding a stress inducible rd29A promoter;
- (b) regenerating transgenic plants from said transformed plant cells;
- and
- (c) selecting a transformed plant from said transgenic plants which exhibits increase in tolerance to an environmental stress as compared to an untransformed plant of the same species, and wherein said increase in environmental stress tolerance is due to the increased expression of the PYL9 protein in the selected transformed plant, wherein the increased expression of the PYL9 polypeptide interacts with abscisic acid (ABA) thereby activating enhanced drought resistance and senescence relative to the control plant.
A method of producing a transgenic plant with increased drought tolerance and leaf senescence as compared to a control or wild type plant, the method comprises the steps of;
- (i) providing a recombinant DNA construct comprising a DNA polynucleotide sequence encoding a PYL9 polypeptide having an amino acid sequence of SEQ ID NO. 104, and wherein the DNA polynucleotide sequence encoding a PYL9 polypeptide is operably linked to a polynucleotide sequence of a stress inducible rd29A promoter, and
- (ii) introducing the recombinant DNA construct into a plant to produce a pRD29A::PYL9 transgenic plant, wherein the pRD29A::PYL9 transgenic plant produces an overexpression of the PYL9 polypeptide to interact with abscisic acid (ABA) thereby exhibiting increased drought tolerance and leaf senescence relative to the control or wild-type plant.
Dependencies are inferred from the claim text.