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US 10,975,356 B2Granted
Method for obtaining female germline stem cells from follicular aspirates
AssigneeShanghai Jiaotong University
InventorJi Wu
Life of this patent
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Abstract
Provided is a method for obtaining female germline stem cells from follicular aspirates.
Claims12
A method for separating female germline stem cells from follicular aspirates, comprising:
- (a) separating ovarian tissues and/or cell masses from the follicular aspirates and dispersing the ovarian tissues and/or cell masses to obtain single cells;
- and
- (b) inoculating and culturing the single cells obtained in step (a) on inactivated feeder layer cells, and sorting female germline stem cell marker-positive cells from the cultured cells.
Dependencies are inferred from the claim text.