Genetically modified microorganism and method for producing indigo dye
AssigneeIndustrial Technology Research Institute
InventorsPei-Ching Chang, Jhong-De Lin, Chang-Jung Chang, Ya-Lin Lin, Hsiang-Yuan Chu, Jie-Len Huang
Life of this patent
Estimating the term…
Abstract
A genetically modified microorganism includes: an exogenous nucleic acid sequence encoding naphthalene dioxygenase (NDO), wherein the endogenous icd gene of the genetically modified microorganism is knocked out, in which the endogenous icd gene encodes isocitrate dehydrogenase (IDH), and wherein the genetically modified microorganism is capable of using glutamic acid and/or a salt thereof as a nitrogen source to grow and producing indigo dye.
Claims20
A genetically modified microorganism, comprising:
- an exogenous nucleic acid sequence encoding naphthalene dioxygenase (NDO),
- wherein the endogenous icd gene of the genetically modified microorganism is knocked out, and the endogenous icd gene encodes isocitrate dehydrogenase (IDH), and
- wherein the genetically modified microorganism is capable of using glutamic acid and/or its salts as a nitrogen source to grow and produce indigo dye.
A novel genetically modified strain of Escherichia coli of which the deposit number is DSM 33021, wherein the endogenous icd gene of the novel genetically modified strain of Escherichia coli is knocked out, and the novel genetically modified strain of Escherichia coli comprises an exogenous nucleic acid sequence encoding naphthalene dioxygenase.
Dependencies are inferred from the claim text.