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US 10,973,927 B2Granted
Materials and methods for effective in vivo delivery of DNA nanostructures to atherosclerotic plaques
AssigneeThe Chinese University of Hong Kong
InventorsChung Hang Jonathan Choi, Lei Zhang
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Abstract
Provided are DNA-coated nanoparticles (DNA-NPS), superparamagnetic nanoparticles (DNA-SPNs), and superparamagnetic iron oxide nanopa rticles (DNA-SPIONs) as efficient imaging agents for targeting and imaging atherosclerotic lesions and treating atherosclerotic disease. The DNA-NS, DNA-SPNs, and DNA-SPIONs can enter macrophage cells via the Class A scavenger receptor (SR-A)-mediated pathways and can be used to specifically target atheroscleortic plaques.
Claims8
A process of using a nanoparticle (NP)-cored spherical nucleic acid (DNA-NP) comprising the steps of:
- obtaining a DNA-NP comprising poly(ethylene glycol)(PEG)-linked oligonucleotides wherein the obtaining step comprises:
- synthesizing an iron core-poly(ethylenglycol)(PEG)-oleylamine NP using PEG diacid and attaching at least one amino-modified DNA oligonucleotide to the iron core-PEG-oleylamine NP, wherein the iron core-PEG-oleylamine NP is devoid of other polymerized structures in the layers between the iron core and the DNA oligonucleotides;
- and
- manufacturing a product comprising said DNA-NP comprising PEG-linked oligonucleotides, wherein the process further comprises loading the DNA-NP comprising PEG-linked oligonucleotides with at least one therapeutic agent.
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Dependencies are inferred from the claim text.