Compositions and methods for improving mitochondrial function
AssigneeThe General Hospital Corporation
InventorsGary Ruvkun, J. Amaranath Govindan, Elamparithi JAYAMANi
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Abstract
Provided herein are methods and compositions comprising a bacterium or a metabolite thereof for enhancing mitochondrial and/or peroxisomal function.
Claims27
A composition comprising a measured amount of a bacterial species that comprises and expresses nucleic acid sequences encoding membrane-bound PQQ-dependent glucose dehydrogenase (mGDH), ubiquinol-cytochrome c reductase iron-sulfur subunit, TonB-dependent receptor, carbon-nitrogen hydrolase, and ubiquinol oxidase subunit II, or an extract or fraction derived therefrom,
- wherein the extract or fraction of the bacterial species comprises membrane-bound PQQ-dependent glucose dehydrogenase (mGDH), ubiquinol-cytochrome c reductase iron-sulfur subunit, TonB-dependent receptor, carbon-nitrogen hydrolase, and ubiquinol oxidase subunit II expressed from the nucleic acid sequences,
- wherein the bacterial species is from the Acetobacteriaceae family, and
- wherein one or more of the nucleic acid sequences are exogenous nucleic acid sequences.
A method for making a bacterial extract, the method comprising culturing a bacterial species that comprises and expresses nucleic acid sequences encoding membrane-bound PQQ-dependent glucose dehydrogenase (mGDH), ubiquinol-cytochrome c reductase iron-sulfur subunit, TonB-dependent receptor, carbon-nitrogen hydrolase, and ubiquinol oxidase subunit II
- wherein the bacterial species is from the Acetobacteriaceae family, and wherein one or more of the nucleic acid sequences are exogenous nucleic acid sequences,
- in a medium comprising:
- (i) standard Lysogeny broth (1% tryptone, 0.5% yeast extract, and 1% sodium chloride) containing 1% glucose,
- (ii) standard Hestrin-Schramm broth (D-glucose 2%, 0.5% yeast extract, 0.5% peptone, 0.27% disodium phosphate, 0.115% citric acid) or
- (iii) CaCO3 medium comprising 8% glucose, 0.5% yeast extract, 0.2% mannitol, 0.05% magnesium sulphate, and 10% calcium carbonate.
Dependencies are inferred from the claim text.