Adenoviral vector system for gene delivery
AssigneePurdue Research Foundation
InventorSuresh Kumar Mittal
Life of this patent
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Abstract
Disclosed herein a unique cell line system to generate a novel bovine adenovirus vector that provides more gene insertion capabilities and better immunogenicity for inserted antigens. The unique cell line is used for generating and growing of the new adenovirus vectors for gene delivery or recombinant vaccine production.
Claims5
A vaccine production system comprising a bovine cell line co-transfected with
- a. human adenovirus (HAdV) E1 region;
- b. a bovine adenovirus E1BL gene;
- and
- c. a bovine adenovirus type 3 (BAdV3) vector with E1A, E1BS, E1BL and E3 regions replaced by an antigenic gene.
The bovine adenovirus type 3 (BAdV3) vector with E1A, E1BS, E1BL and E3 regions deleted and a foreign gene up to 5.5kb inserted for expression under the control of an effective promoter at the site of deletion, wherein the foreign gene comprises SEQ ID NO:
- 8.
Dependencies are inferred from the claim text.