High temperature seed germination
AssigneeRijk Zwaan Zaadteelt En Zaadhandel B.V.
InventorsEric Roland Coppoolse, Miriam Post
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Abstract
The present invention relates to a seed comprising in its genome a modified NXS gene and/or modified regulatory sequences thereof. The modified NXS gene and/or modified regulatory sequences thereof provides the seed with the capability to germinate at a high temperature as compared to a wild type seed not having the modified NXS gene. The modification to the gene and/or its regulatory sequences may lead to the expression of the NXS gene being substantially reduced or prevented. In addition to or alternatively, the seed can have a reduced level, reduced activity or complete absence of NXS protein. The modified NXS gene may for example comprise a premature stop codon and/or encode an NXS protein that comprises one or more amino acid substitutions.
Claims25
A non-naturally occurring lettuce seed (Lactuca sativa) comprising
- in its genome homozygously a modified neoxanthin synthase (NXS) gene
- that is non-naturally occurring,
- the modified NXS gene comprising a premature stop codon, with reference to SEQ ID NO:
- 1 that encodes neoxanthin synthase, with reference to SEQ ID NO:
- 2, whereby the premature stop codon results in loss of neoxanthin synthase function,
- wherein the seed germinates at a high temperature as compared with a wild type seed not having the modified NXS gene,
- said high temperature comprising at least 29° C. to 40° C.
A lettuce plant (Lactuca sativa) comprising
- in its genome a modified neoxanthin synthase (NXS) gene
- that is non-naturally occurring,
- the modified NXS gene comprising a premature stop codon, with reference to SEQ ID NO:
- 1 that encodes neoxanthin synthase, with reference to SEQ ID NO:
- 2, whereby the premature stop codon results in loss of neoxanthin synthase function,
- wherein seed of the plant having the modified NXS gene homozygously germinates at a high temperature as compared with a seed of a wild type plant not having the modified NXS gene,
- said high temperature comprising 29° C. to 40° C.
Dependencies are inferred from the claim text.