Methods and products for genetic engineering
AssigneesInstitut National De La Sante Et De La Recherche Medicale (Inserm), Centre National de la Recherche Scientifique (CNRS), Ecole Normale Superieure De Lyon, Universite Claude Bernard Lyon 1
InventorsThéophile Ohlmann, Philippe Mangeot, Emiliano Ricci
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Abstract
The present invention relates to a virus-derived particle comprising one or more Cas protein(s), as well as to kits and methods using the same for altering a target nucleic acid.
Claims24
A retrovirus-derived particle comprising one or more Cas protein(s) wherein the one or more Cas protein is contained within an inside of said particle as a fusion protein between (i) a viral structural protein and (ii) the one or more Cas protein(s), wherein the retrovirus-derived particle is devoid of any encoding nucleic acid, and wherein the one or more Cas protein(s) is complexed with one or more CRISPR-Cas system guide RNA(s).
A kit for preparing retrovirus-derived particles for altering a target nucleic acid in a eukaryotic cell comprising:
- a nucleic acid comprising an expression cassette encoding a GAG-Cas fusion protein,
- a nucleic acid comprising one or more expression cassette(s) encoding virus-derived assembly protein(s), and
- one or more nucleic acid(s) encoding a CRISPR-Cas system Guide RNA.
Dependencies are inferred from the claim text.