Wide-angle emission filter, optical sensor assembly having the same, PCR system having the same, and method of manufacturing the same

An optical sensor assembly comprising:

• a wide-angle emission filter configured to filter an excitation light regardless of an incident angle of the excitation light and to transmit emission light having a wavelength greater than the excitation light, the wide-angle emission filter including:
• a base matrix having a flat shape and including a transparent material, the base matrix not generating fluorescent light or phosphorescent light by the excitation light;
• a photoresist disposed in the base matrix, the photoresist being fixed in a solid state through at least one method selected from the group consisting of thermal hardening, photo hardening, and drying;
• and
• a colorant disposed in the base matrix and including light having a predetermined wavelength range;
• and
• an optical sensor substrate including:
• a base substrate having a flat shape and being integrally formed with the wide-angle emission filter;
• and
• an optical sensor array including a plurality of optical sensors buried in an upper portion of the base substrate and arranged in an array shape to sense luminance of the emission light having passed through the wide-angle emission filter,
• wherein the photoresist comprises a semi-solidified photoresist, which is not completely saturated by light having a short wavelength, and the excitation light is firstly filtered by the colorant, and wherein the semi-solidified photoresist is configured to secondly filter the excitation light to form a secondly filtered semi-solidified photoresist, and
• wherein the photoresist is mixed with the colorant and is disposed in the base matrix, and wherein the photoresist, the colorant, and the base matrix form a same layer.

A polymerase chain reaction (PCR) system comprising:

• a PCR module including:
• a wide-angle emission filter configured to filter an excitation light regardless of an incident angle of the excitation light, the wide-angle emission filter including a photoresist fixed in a solid state through at least one method selected from the group consisting of thermal hardening, photo hardening, and drying;
• and a colorant including light having a predetermined wavelength range;
• an optical sensor substrate including an optical sensor array including a plurality of optical sensors arranged in an array shape to sense luminance of the emission light having passed through the wide-angle emission filter to generate a optical sensing signal;
• a reaction space disposed on the wide-angle emission filter to receive a specimen, in which PCR is performed;
• and
• a first temperature controlling part receiving a temperature control signal to control temperature in the reaction space;
• and
• a reader system including:
• a central processing unit receiving the optical sensing signal to calculate an amount of gene amplification based on the optical sensing signal to generate the temperature control signal;
• a light source generating the excitation light;
• and
• a second temperature controlling part connected to the central processing unit to control temperature of the PCR module,
• wherein the photoresist comprises a semi-solidified photoresist, which is not completely saturated by light having a short wavelength, and the excitation light is firstly filtered by the colorant, and wherein the semi-solidified photoresist is configured to secondly filter the excitation light to form a secondly filtered semi-solidified photoresist, and
• wherein the photoresist is mixed with the colorant and is disposed in a base matrix, and wherein the photoresist, the colorant, and the base matrix form a same layer.