Method for the fermentative production of molecules of interest by microorganisms comprising genes coding sugar phosphotransferase system (PTS)

A method for the production of a molecule of interest by conversion of a source of carbon in a fermentative process comprising the following steps:

• culturing a microorganism genetically modified for the production of the molecule of interest in an appropriate culture medium comprising a carbohydrate as source of carbon and
• recovering the molecule of interest from the culture medium,
• wherein said genetically modified microorganism comprises functional genes coding for a phosphotransferase carbohydrate utilization system,
• wherein in said genetically modified microorganism the expression of the bifunctional ADP-dependent kinase-Pi-dependent pyrophosphorylase protein regulating the expression of the phosphoenolpyruvate synthase (PPS) is decreased,
• wherein the molecule of interest is selected from glycolic acid, lactic acid, 1,3-propanediol, 1,2-propanediol, prenol, isobutene, butadiene, butanediol, methyl ethyl ketone, succinic acid, glutamic acid, adipic acid, muconic acid, lysine, dodecanedioic acid, farnesene, and 2,4-dihydroxybutyric acid, and
• wherein the carbohydrate is selected from the group consisting of glucose, fructose, mannose, cellobiose, and sucrose, and any combination thereof.