Multiplexed genome editing
AssigneePresident and Fellows of Harvard College
InventorsGeorge M. Church, Luhan Yang, Marc Guell
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Abstract
A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.
Claims8
A method of inactivating expression of at least 20% of the copies of an endogenous retroviral target gene in a cell comprising multiple copies of the gene, comprising:
- (a) introducing into the cell (i) one or more guide ribonucleic acid (gRNA) sequences that comprise a portion that is complementary to a portion of the endogenous retroviral target gene, and (ii) a nucleic acid sequence that encodes a Cas protein;
- and
- (b) maintaining the cells under conditions in which (i) the Cas protein is expressed, (ii) the Cas protein and gRNAs form co-localization complexes with a plurality of the copies of the endogenous retroviral target gene, and (iii) the Cas protein cuts at least 20% of the copies of the endogenous retroviral target gene, thereby inactivating expression of said copies.
Dependencies are inferred from the claim text.