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US 10,955,424 B2Granted
Mass spectrometry of steroidal compounds in multiplexed patient samples
AssigneeQuest Diagnostics Investments Incorporated
InventorsBrett Holmquist, Nigel Clarke
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Abstract
The invention relates to the quantitative measurement of steroidal compounds by mass spectrometry. In a particular aspect, the invention relates to methods for quantitative measurement of steroidal compounds from multiple samples by mass spectrometry.
Claims21
A method for determining the amount of one or more vitamin D metabolites in each of a plurality of human samples with a single mass spectrometric assay, the method comprising:
- i) subjecting each of a plurality of human samples to a different Cookson-type derivatizing agent to generate a differently derivatized one or more vitamin D metabolites in each of the plurality of samples;
- ii) combining the plurality of samples to form a multiplex sample;
- and
- iii) quantifying the amount of the one or more vitamin D metabolites in each sample by mass spectrometry, wherein the method has a limit of quantitation (LOQ) within the range of 1.9 ng/mL to 10 ng/mL, inclusive.
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Dependencies are inferred from the claim text.