US 10,953,085 B2 · Genetically engineered Foot and Mouth Disease Virus and related proteins, polynucleotides, compositions, methods and systems

Genetically engineered Foot and Mouth Disease Virus and related proteins, polynucleotides, compositions, methods and systems

AssigneesLawrence Livermore National Security, LLC, The United States of America as represented by the Secretary of Agriculture, University of Connecticut

InventorsAida E. Rieder, Teresa B. De Los Santos, Luis L. Rodriguez, Devendra Rai, Fayna C. Diaz-San Segundo, Paul D. Hoeprich

Life of this patent

Estimating the term…

Abstract

Genetically engineered Foot and Mouth Disease Virus (FMDV) and related engineered proteins and polynucleotides, nanolipoprotein particles, compositions, methods and systems are described. The genetically engineered FMDV is modified by the strategic insertion of a protein tag into select regions of the FMDV genome which encode viral proteins that are exposed on the surface of the FMDV viral capsid. The inserted protein tag is displayed as a decoration or attachment on the viral capsid surface.

Claims33

Dependent claims

A genetically modified Foot and Mouth Disease Virus (FMDV) having a viral capsid comprising an engineered FMDV VP1 protein, the engineered FDMV VP1 protein comprising

an FMDV VP1 protein having an FMDV VP1 N-terminus and an FMDV VP1 C-terminus,
a protein tag having up to 100 amino acids, and
an FMDV 2A protein having an FMDV 2A N-terminus and an FMDV 2A C-terminus, wherein
the FMDV VP1 C-terminus is fused to the FMDV 2A N-terminus and
the protein tag is inserted in the FMDV VP1 protein at an insertion position selected
from any one of position −1 to position +6 relative to the FMDV 2A N-terminus, or
from any one of position −1 to position −7 relative to the FMD VP1 C-terminus,
and wherein the protein tag is presented on an external surface of the viral capsid.

5 more elements

Dependencies are inferred from the claim text.