Material and method for colorimetric detection of small-molecule targets
AssigneeThe Florida International University Board of Trustees
InventorsYi Xiao, Yingping Luo, Haixiang Yu
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Abstract
The subject invention provides methods, assays, and products for visual detection of small-molecule targets in a sample in both clinical and field settings within minutes. The subject invention is based on an aptamer sensor that reports the presence of small-molecule target via a sensitive colorimetric signal for naked-eye detection. The aptamer sensor is a CBSAzyme-based sensor having both target-mediated cooperative behavior of the CBSA and peroxidase-mimicking catalytic activity of DNAzyme. The subject invention also provides methods of using the CBSAzyme-based sensor.
Claims20
A cooperative binding split aptamer (CBSA)-DNAzyme conjugate (CBSAzyme) comprising a first fragment and a second fragment, the first fragment comprising a first segment of a split DNAzyme and a long fragment of a CBSA, and the second fragment comprising a second segment of the split DNAzyme and a short fragment of the CBSA, the first and second segments of the split DNAzyme being split from a DNAzyme having a G-quadruplex-structure and peroxidase activity, two fragments of CBSAzyme assembling upon binding of a target molecule of the CBSA.
Dependencies are inferred from the claim text.