Mass spectrometry assay for congenital adrenal hyperplasia
AssigneeQuest Diagnostics Investments Incorporated
InventorsAmit Ghoshal, Nigel J. Clarke, Mildred M. Goldman
Life of this patent
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Abstract
Methods are provided for detecting the amount of one or more CAH panel analytes (i.e., pregnenolone, 17-OH pregnenolone, progesterone, 17-OH progesterone, dehydroepiandrosterone (DHEA), androstenedione, testosterone, deoxycorticosterone, 11-deoxycortisol, and cortisol) in a sample by mass spectrometry. The methods generally involve ionizing one or more CAH panel analytes in a sample and quantifying the generated ions to determine the amount of one or more CAH panel analytes in the sample. In methods where amounts of multiple CAH panel analytes are detected, the amounts of multiple analytes are detected in the same sample injection.
Claims7
A method for determining the amount of 17-OH pregnenolone in a sample by mass spectrometry, the method comprising:
- (i) purifying the sample by liquid chromatography;
- (ii) ionizing the sample under conditions suitable to produce one or more 17-OH pregnenolone ions detectable by mass spectrometry, wherein the one or more 17-OH pregnenolone ions comprise one or more ions having a m/z of about 297.2±0.5, 105.6±0.5, and/or 91.1±0.5;
- (iii) determining the amount of the one or more 17-OH pregnenolone ions by mass spectrometry, wherein the method has a limit of quantitation of 6 ng/dL;
- and
- (iv) using the determined amount of the one or more ions to determine the amount of 17-OH pregnenolone in the sample.
Dependencies are inferred from the claim text.