Methods of assaying proteins
AssigneeNautilus Biotechnology, Inc.
InventorParag Mallick
Life of this patent
Estimating the term…
Abstract
Methods and systems for identifying a protein within a sample are provided herein. A panel of antibodies are acquired, none of which are specific for a single protein or family of proteins. Additionally, the binding properties of the antibodies in the panel are determined. Further, the protein is iteratively exposed to a panel of antibodies. Additionally, a set of antibodies which bind the protein are determined. The identity of the protein is determined using one or more deconvolution methods based on the known binding properties of the antibodies to match the set of antibodies to a sequence of a protein.
Claims29
A system for identifying polypeptide molecules, comprising:
- a plurality of polypeptide molecules, each polypeptide molecule of said plurality of polypeptide molecules being coupled to a unique, spatially-separated location of a plurality of unique, spatially-separated locations on a substrate;
- a plurality of affinity reagent pools, wherein at least one affinity reagent pool of said plurality of affinity reagent pools binds to more than one unique epitope present within one or more polypeptides in said plurality of polypeptide molecules when said at least one affinity reagent pool is exposed to said substrate and wherein at least one affinity reagent of said plurality of affinity reagent pools comprises a known degree of binding nonspecificity;
- a detection system for detecting a pattern of interaction of said plurality of affinity reagent pools with individual polypeptide molecules of said plurality of polypeptide molecules at said plurality of unique, spatially-separated locations on said substrate;
- and
- an analysis system for characterizing said individual polypeptide molecules of said plurality of polypeptide molecules based upon a pattern of interaction detected by said detection system.
Dependencies are inferred from the claim text.