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US 10,948,487 B2Granted
Peptides, devices, and methods for the detection of Ehrlichia antibodies
AssigneeZoetis Services LLC
InventorsRajesh K. Mehra, Kenneth P. Aron, Dennis M. Bleile, Timothy P. Forsyth, Jeremy D. Walker, Cristina R. Cuesico
Life of this patent
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Abstract
The invention provides peptide compositions and mixtures useful for the detection of antibodies that bind to Ehrlichia antigens. The peptide compositions and mixtures comprise polypeptide sequences based on an immunogenic fragment of the Ehrlichia Outer Membrane Protein 1 (OMP-1) protein. The invention also provides devices, methods, and kits comprising such peptide compositions and mixtures useful for the detection of antibodies that bind to Ehrlichia antigens and the diagnosis of monocytic and/or granulocytic ehrlichiosis.
Claims26
A population of isolated peptides comprising two or more different peptides, the population of peptides comprising:
- (i) one or more peptides comprising the sequence of S-X2-K-E-X5-K-Q-X8-T-X10-X11-X12-X13-G-L-K-Q-X18-W-X20-G-X22-X23-X24-X25-X26-G-G-G-G-G-N-F-S-A-K-E-E-X39-A-E-T R-X44-T-F-G-L-X49-K-Q-Y-D-G-A-X56-I-X58-E-N-Q-V-Q-N-K-F-T-I-S-N-C(SEQ ID NO:72) or fragment thereof comprising at least 20 contiguous amino acids of SEQ ID NO:
- 72, wherein X2 is an amino acid selected from the group consisting of A and V, X5 is an amino acid selected from the group consisting of E and D, X8 is an amino acid selected from the group consisting of T and P, X10 is an amino acid selected from the group consisting of T and V, X11 is an amino acid selected from the group consisting of G and A, X12 is an amino acid selected from the group consisting of L and V, X13 is an amino acid selected from the group consisting of Y and F, X18 is an amino acid selected from the group consisting of D and N, X20 is an amino acid selected from the group consisting of D and N, X22 is an amino acid selected from the group consisting of S and V, X23 is an amino acid selected from the group consisting of A, S, and T, X24 is an amino acid selected from the group consisting of A and I, X25 is an amino acid selected from the group consisting of T and P, X26 is an amino acid selected from the group consisting of S, N, and K, X39 is any amino acid, X44 is any amino acid, X49 is any amino acid, X56 is any amino acid, and X58 is any amino acid,
- (ii) one or more peptides comprising the sequence of S-X2-K-E-X5-K-Q-X8-T-X10-X11-X12-X13-G-L-K-Q-X18-W-X20-G-X22-X23-X24-X25-X26-G-G-G-G-G-N-F-S-A-K-E-E-X39-A-X41-T-R-X44-T-F-G-X48-X49-K-Q-Y-D-G-A-X56-I-X58-E-N-Q-V-Q-N-K-F-T-I-S-N-C(SEQ ID NO:
- 3) or a fragment thereof comprising at least 20 contiguous amino acids of SEQ ID NO:
- 3, wherein X2 is an amino acid selected from the group consisting of A and V, X5 is an amino acid selected from the group consisting of E and D, X7 is any amino acid, X8 is an amino acid selected from the group consisting of T and P, X10 is an amino acid selected from the group consisting of T and V, X11 is an amino acid selected from the group consisting of G and A, X12 is an amino acid selected from the group consisting of L and V, X13 is an amino acid selected from the group consisting of Y and F, X18 is an amino acid selected from the group consisting of D and N, X20 is an amino acid selected from the group consisting of D and N, X22 is an amino acid selected from the group consisting of S and V, X23 is an amino acid selected from the group consisting of A, S, and T, X24 in SEQ ID NOs:
- 72 and is an amino acid selected from the group consisting of A and I, X25 is an amino acid selected from the group consisting of T and P, X26 is an amino acid selected from the group consisting of S, N, and K, X39 is any amino acid, X41 is an amino acid selected from the group consisting of D and N, X44 is any amino acid, X48 is an amino acid selected from the group consisting of V and A, X49 is any amino acid, X56 is any amino acid, and X58 is any amino acid,
- (iii) one or more peptides comprising the sequence of F-S-A-K-E-E-X7-A-E-T-R-X12-T-F-G-L-X17-K-Q-Y-D-G-A-X24-I-X26-E-N-Q-V-Q-N-K-F-T-I-S-N-C(SEQ ID NO:
- 71) or a fragment thereof comprising at least 20 contiguous amino acids of SEQ ID NO:
- 71, wherein X7 is any amino acid, X12 is any amino acid, X17 is any amino acid, X24 is any amino acid, and X26 is any amino acid, or
- (iv) one or more peptides from each of (i) and (ii), one or more peptides from each of (i) and (iii), one or more peptides from each of (ii) and (iii), or one or more peptides from each of (i), (ii), and (iii).
7 more elements
A population of isolated peptides comprising
- (i) one or more peptides comprising the sequence of S-X2-K-E-X5-K-Q-X8-T-X10-X11-X12-X13-G-L-K-Q-X18-W-X20-G-X22-X23-X24-X25-X26-G-G-G-G-G-N-F-S-A-K-E-E-X39-A-E-T-R-X44-T-F-G-L-X49-K-Q-Y-D-G-A-X56-I-X58-E-N-Q-V-Q-N-K-F-T-I-S-N-C(SEQ ID NO:72) or fragment thereof comprising at least 20 contiguous amino acids of SEQ ID NO:
- 72, wherein X2 is an amino acid selected from the group consisting of A and V, X5 is an amino acid selected from the group consisting of E and D, X8 is an amino acid selected from the group consisting of T and P, X10 is an amino acid selected from the group consisting of T and V, X11 is an amino acid selected from the group consisting of G and A, X12 is an amino acid selected from the group consisting of L and V, X13 is an amino acid selected from the group consisting of Y and F, X18 is an amino acid selected from the group consisting of D and N, X20 is an amino acid selected from the group consisting of D and N, X22 is an amino acid selected from the group consisting of S and V, X23 is an amino acid selected from the group consisting of A, S, and T, X24 is an amino acid selected from the group consisting of A and I, X25 is an amino acid selected from the group consisting of T and P, X26 is an amino acid selected from the group consisting of S, N, and K, X39 is any amino acid, X44 is any amino acid, X49 is any amino acid, X56 is any amino acid, and X58 is any amino acid,
- (ii) one or more peptides comprising the sequence of S-X2-K-E-X5-K-Q-X8-T-X10-X11-X12-X13-G-L-K-Q-X18-W-X20-G-X22-X23-X24-X25-X26-G-G-G-G-G-N-F-S-A-K-E-E-X39-A-X41-T-R-X44-T-F-G-X48-X49-K-Q-Y-D-G-A-X56-I-X58-E-N-Q-V-Q-N-K-F-T-I-S-N-C(SEQ ID NO:
- 3) or a fragment thereof comprising at least 20 contiguous amino acids of SEQ ID NO:
- 3, wherein X2 is an amino acid selected from the group consisting of A and V, X5 is an amino acid selected from the group consisting of E and D, X7 is any amino acid, X8 is an amino acid selected from the group consisting of T and P, X10 is an amino acid selected from the group consisting of T and V, X11 is an amino acid selected from the group consisting of G and A, X12 is an amino acid selected from the group consisting of L and V, X13 is an amino acid selected from the group consisting of Y and F, X18 is an amino acid selected from the group consisting of D and N, X20 is an amino acid selected from the group consisting of D and N, X22 is an amino acid selected from the group consisting of S and V, X23 is an amino acid selected from the group consisting of A, S, and T, X24 in SEQ ID NOs:
- 72 and is an amino acid selected from the group consisting of A and I, X25 is an amino acid selected from the group consisting of T and P, X26 is an amino acid selected from the group consisting of S, N, and K, X39 is any amino acid, X41 is an amino acid selected from the group consisting of D and N, X44 is any amino acid, X48 is an amino acid selected from the group consisting of V and A, X49 is any amino acid, X56 is any amino acid, and X58 is any amino acid, and
- (iii) one or more peptides comprising the sequence of F-S-A-K-E-E-X7-A-E-T-R-X12-T-F-G-L-X17-K-Q-Y-D-G-A-X24-I-X26-E-N-Q-V-Q-N-K-F-T-I-S-N-C(SEQ ID NO:
- 71) or a fragment thereof comprising at least 20 contiguous amino acids of SEQ ID NO:
- 71, wherein X7 is any amino acid, X12 is any amino acid, X17 is any amino acid, X24 is any amino acid, and X26 is any amino acid.
6 more elements
Dependencies are inferred from the claim text.