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US 10,947,603 B2Granted
HPV detection method
AssigneeCellCall KFT.
InventorTibor Takacs
Life of this patent
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Abstract
This invention relates to the detection of human papillomavirus (HPV) genotypes, particularly genital human papillomavirus genotypes, using PCR related methods.
Claims24
A method for detecting the presence of HPV, comprising amplifying a nucleic acid obtained from a sample utilizing one or more amplification mixtures, wherein the amplification mixtures are selected from:
- (a) Amplification mixture 1 comprising
- (SEQ. ID NO:
- 1)
- 5′-TCATGCAGGAACATCCAGACT-3′
- (SEQ. ID NO:
- 2)
- 5′-CCAAACTTATTGGGGTCAGG-3′
- (SEQ. ID NO:
- 3)
- 5′-TTGCAGTTGGACATCCCTATTTTCC-3′
- (b) Amplification mixture 2 comprising
- (SEQ. ID NO:
- 4)
- 5′-TCATGCTGGCAGCTCTAGATT-3′
- (SEQ. ID NO:
- 5)
- 5′-GGTCAGGTAACTGCACCCTAAA-3′
- (SEQ. ID NO:
- 6)
- 5′-AGGGTTCCTGCAGGTGGTGGC-3′
- (c) Amplification mixture 3 comprising
- (SEQ. ID NO:
- 10)
- 5′-CACGCAGGCAGTGCTAGG-3′
- (SEQ. ID NO:
- 11)
- 5′-TCCAAATTTGTTTGGATCTGG-3′
- (SEQ. ID NO:
- 12)
- 5′-CAGTAGGCCATCCATATTATTCCATACC-3′
- (d) Amplification mixture 4 comprising
- (SEQ. ID NO:
- 13)
- 5′-GCTGGTAGTTCCAGACTTCTTGC-3′
- (SEQ. ID NO:
- 14)
- 5′-CCAAATTTATTAGGATCTGGTAAACG-3′
- (SEQ. ID NO:
- 15)
- 5′-TGGTACCCAAAGTATCAGGCTTGCA-3′
- (e) Amplification mixture 5 comprising
- (SEQ. ID NO:
- 16)
- 5′-TTATGCAGGCAGTTCTCGATT-3′
- (SEQ. ID NO:
- 17)
- 5′-GGGTCCGGCAATTTAATTCT-3′
- (SEQ. ID NO:
- 18)
- 5′-AGGACATCCCTATTTTTCTATTAAAAACACCAGT-3′
- (f) Amplification mixture 6 comprising
- (SEQ. ID NO:
- 19)
- 5′-TATCATGCAGGCAGTTCACG-3′
- (SEQ. ID NO:
- 20)
- 5′-AAACTTATTAGGGTCGGGCAAC-3′
- (SEQ. ID NO:
- 21)
- 5′-GGACAATACCAAAACAAACATTCCCA-3′
- (g) Amplification mixture 7 comprising
- (SEQ. ID NO:
- 22)
- 5′-TGCTGGCAGTTCCAGACTTT-3′
- (SEQ. ID NO:
- 23)
- 5′-AAACCAAATTTATTGGGATCAGG-3′
- (SEQ. ID NO:
- 24)
- 5′-TCCCAAGGTATCAGGCTTACAGTATAGGG-3′
- (h) Amplification mixture 8 comprising
- (SEQ. ID NO:
- 25)
- 5′-ACGCAGGCAGTTCCAGACT-3′
- (SEQ. ID NO:
- 26)
- 5′-GGCCAAATTTATTGGGATCA-3′
- (SEQ. ID NO:
- 27)
- 5′-TGGTAGACAGGATGTTCCTAAGGTGTC-3′;
- or
- (i) Amplification mixture 9 comprising
- (SEQ. ID NO:
- 31)
- 5′-TCATGCAGGCAGTTCTAGGC-3′
- (SEQ. ID NO:
- 32)
- 5′-GAAATCCAAACTTATTAGGATCTGGT-3′
- (SEQ. ID NO:
- 33)
- 5′-AGCAGTACCCAAGGTATCTGGTTTGCA-3′
- (j) Amplification mixture 10 comprising
- (SEQ. ID NO:
- 34)
- 5′-ATGCTGGCAGCTCTAGATTATT-3′
- (SEQ. ID NO:
- 35)
- 5′-TTAGGATCGGGCAATGTCAC-3′
- (SEQ. ID NO:
- 36)
- 5′-TGAATGGTGGTCGCAAGCAGG-3′
- (k) Amplification mixture 11 comprising
- (SEQ. ID NO:
- 37)
- 5′-GCAGGCAGTTCCCGATTATT-3′
- (SEQ. ID NO:
- 38)
- 5′-TCCAAATTTATTAGGATCGGGTAAA-3′
- (SEQ. ID NO:
- 39)
- 5′-CAG GCT GTT CCT AAG GTA TCC GCA-3′
- (l) Amplification mixture 12 comprising
- (SEQ. ID NO:
- 40)
- 5′-TGCAGGCAGTTCCAGACTAA-3′
- (SEQ. ID NO:
- 41)
- 5′-GAGTCCAAACTTGTTAGGATCTGGT-3′
- (SEQ. ID NO:
- 42)
- 5′-CCTCAACGCGTGCTGCTATTCC-3′
- (m) Amplification mixture 13 comprising
- (SEQ. ID NO:
- 43)
- 5′-TGCAGGTAGCTCTAGGTTGCT-3′
- (SEQ. ID NO:
- 44)
- 5′-TAGGATCAGGCAACCGTACC-3′
- (SEQ. ID NO:
- 45)
- CAAATCTGGTACCAAAACAAACATCCC-3′
- (n) Amplification mixture 14 comprising
- (SEQ. ID NO:
- 46)
- TGCTGGTACATCTAGGTTATTAACTG-3′
- (SEQ. ID NO:
- 47)
- 5′-CAGGAACACTAAATTTATTAGGATCAGG-3′
- (SEQ. ID NO:
- 48)
- 5′-CTATGTCTGGGGGCCGCAAG.-3′;
- Wherein SEQ ID No's:
- 3, 6, 12, 15, 18, 21, 24, 27, 33, 36, 39, 42, 45 and 48 are labelled with a quencher and dye, one at the 5′ end and one at the 3′ end.
140 more elements
A method for detecting the presence of HPV, comprising amplifying a nucleic acid obtained from a sample utilizing one or more amplification mixtures, wherein the amplification mixtures are selected from:
- (a) Amplification mixture 15 comprising
- (SEQ. ID NO:
- 1)
- 5′-TCATGCAGGAACATCCAGACT-3′
- (SEQ. ID NO:
- 2)
- 5′-CCAAACTTATTGGGGTCAGG-3′
- (SEQ. ID NO:
- 3)
- 5′-TTGCAGTTGGACATCCCTATTTTCC-3′
- (SEQ. ID NO:
- 4)
- 5′-TCATGCTGGCAGCTCTAGATT-3′
- (SEQ. ID NO:
- 5)
- 5′-GGTCAGGTAACTGCACCCTAAA-3′
- (SEQ. ID NO:
- 6)
- 5′-AGGGTTCCTGCAGGTGGTGGC-3′
- (b) Amplification mixture 16 comprising
- (SEQ. ID NO:
- 10)
- 5′-CACGCAGGCAGTGCTAGG-3′
- (SEQ. ID NO:
- 11)
- 5′-TCCAAATTTGTTTGGATCTGG-3′
- (SEQ. ID NO:
- 12)
- 5′-CAGTAGGCCATCCATATTATTCCATACC-3′
- (SEQ. ID NO:
- 13)
- 5′-GCTGGTAGTTCCAGACTTCTTGC-3′
- (SEQ. ID NO:
- 14)
- 5′-CCAAATTTATTAGGATCTGGTAAACG-3′
- (SEQ. ID NO:
- 15)
- 5′-TGGTACCCAAAGTATCAGGCTTGCA-3′
- (SEQ. ID NO:
- 16)
- 5′-TTATGCAGGCAGTTCTCGATT-3′
- (SEQ. ID NO:
- 17)
- 5′-GGGTCCGGCAATTTAATTCT-3′
- (SEQ. ID NO:
- 18)
- 5′-AGGACATCCCTATTTTTCTATTAAAAACACCAGT-3′
- (SEQ. ID NO:
- 19)
- 5′-TATCATGCAGGCAGTTCACG-3′
- (SEQ. ID NO:
- 20)
- 5′-AAACTTATTAGGGTCGGGCAAC-3′
- (SEQ. ID NO:
- 21)
- 5′-GGACAATACCAAAACAAACATTCCCA-3′
- (SEQ. ID NO:
- 22)
- 5′-TGCTGGCAGTTCCAGACTTT-3′
- (SEQ. ID NO:
- 23)
- 5′-AAACCAAATTTATTGGGATCAGG-3′
- (SEQ. ID NO:
- 24)
- 5′-TCCCAAGGTATCAGGCTTACAGTATAGGG-3′
- (SEQ. ID NO:
- 25)
- 5′-ACGCAGGCAGTTCCAGACT-3′
- (SEQ. ID NO:
- 26)
- 5′-GGCCAAATTTATTGGGATCA-3′
- (SEQ. ID NO:
- 27)
- 5′-TGGTAGACAGGATGTTCCTAAGGTGTC-3′;
- or
- (c) Amplification mixture 17 comprising
- (SEQ. ID NO:
- 31)
- 5′-TCATGCAGGCAGTTCTAGGC-3′
- (SEQ. ID NO:
- 32)
- 5′-GAAATCCAAACTTATTAGGATCTGGT-3′
- (SEQ. ID NO:
- 33)
- 5′-AGCAGTACCCAAGGTATCTGGTTTGCA-3′
- (SEQ. ID NO:
- 34)
- 5′-ATGCTGGCAGCTCTAGATTATT-3′
- (SEQ. ID NO:
- 35)
- 5′-TTAGGATCGGGCAATGTCAC-3′
- (SEQ. ID NO:
- 36)
- 5′-TGAATGGTGGTCGCAAGCAGG-3′
- (SEQ. ID NO:
- 37)
- 5′-GCAGGCAGTTCCCGATTATT-3′
- (SEQ. ID NO:
- 38)
- 5′-TCCAAATTTATTAGGATCGGGTAAA-3′
- (SEQ. ID NO:
- 39)
- 5′-CAG GCT GTT CCT AAG GTA TCC GCA-3′
- (SEQ. ID NO:
- 40)
- 5′-TGCAGGCAGTTCCAGACTAA-3′
- (SEQ. ID NO:
- 41)
- 5′-GAGTCCAAACTTGTTAGGATCTGGT-3′
- (SEQ. ID NO:
- 42)
- 5′-CCTCAACGCGTGCTGCTATTCC-3′
- (SEQ. ID NO:
- 43)
- 5′-TGCAGGTAGCTCTAGGTTGCT-3′
- (SEQ. ID NO:
- 44)
- 5′-TAGGATCAGGCAACCGTACC-3′
- (SEQ. ID NO:
- 45)
- CAAATCTGGTACCAAAACAAACATCCC-3′
- (SEQ. ID NO:
- 46)
- TGCTGGTACATCTAGGTTATTAACTG-3′
- (SEQ. ID NO:
- 47)
- 5′-CAGGAACACTAAATTTATTAGGATCAGG-3′
- (SEQ. ID NO:
- 48)
- 5′-CTATGTCTGGGGGCCGCAAG.-3′;
- Wherein SEQ ID No's:
- 3, 6, 12, 15, 18, 21, 24, 27, 33, 36, 39, 42, 45 and 48 are labelled with a quencher and dye, one at the 5′ end and one at the 3′ end.
129 more elements
An amplification mixture for the use in the detection of HPV, said amplification mixture comprising
- (a) SEQ. ID No's:
- 1-3;
- and optionally SEQ. ID No's:
- 7-9
- (b) SEQ. ID No's:
- 3-6;
- and optionally SEQ. ID No's:
- 7-9
- (c) SEQ. ID No's:
- 10-12;
- and optionally SEQ. ID No's:
- 28-30
- (d) SEQ. ID No's:
- 13-15;
- and optionally SEQ. ID No's:
- 28-30
- (e) SEQ. ID No's:
- 16-18;
- and optionally SEQ. ID No's:
- 28-30
- (f) SEQ. ID No's:
- 19-21;
- and optionally SEQ. ID No's:
- 28-30
- (g) SEQ. ID No's:
- 22-24;
- and optionally SEQ. ID No's:
- 28-30
- (h) SEQ. ID No's:
- 25-27;
- and optionally SEQ. ID No's:
- 28-30
- (i) SEQ. ID No's:
- 31-33;
- and optionally SEQ. ID No's:
- 49-51
- (j) SEQ. ID No's:
- 34-36;
- and optionally SEQ. ID No's:
- 49-51
- (k) SEQ. ID No's:
- 37-39;
- and optionally SEQ. ID No's:
- 49-51
- (l) SEQ. ID No's:
- 40-42;
- and optionally SEQ. ID No's:
- 49-51
- (m) SEQ. ID No's:
- 43-45;
- and optionally SEQ. ID No's:
- 49-51
- (n) SEQ. ID No's:
- 46-48;
- and optionally SEQ. ID No's:
- 49-51
- Wherein SEQ ID No's:
- 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48 and 51 are labelled with a quencher and dye, one at the 5′ end and one at the 3′ end.
55 more elements
A kit for the use in the detection of HPV comprising one or more amplification mixtures selected from
- a) SEQ. ID No's:
- 1-3;
- and optionally SEQ. ID No's:
- 7-9
- (b) SEQ. ID No's:
- 3-6;
- and optionally SEQ. ID No's:
- 7-9
- (c) SEQ. ID No's:
- 10-12;
- and optionally SEQ. ID No's:
- 28-30
- (d) SEQ. ID No's:
- 13-15;
- and optionally SEQ. ID No's:
- 28-30
- (e) SEQ. ID No's:
- 16-18;
- and optionally SEQ. ID No's:
- 28-30
- (f) SEQ. ID No's:
- 19-21;
- and optionally SEQ. ID No's:
- 28-30
- (g) SEQ. ID No's:
- 22-24;
- and optionally SEQ. ID No's:
- 28-30
- (h) SEQ. ID No's:
- 25-27;
- and optionally SEQ. ID No's:
- 28-30
- (i) SEQ. ID No's:
- 31-33;
- and optionally SEQ. ID No's:
- 49-51
- (j) SEQ. ID No's:
- 34-36;
- and optionally SEQ. ID No's:
- 49-51
- (k) SEQ. ID No's:
- 37-39;
- and optionally SEQ. ID No's:
- 49-51
- (l) SEQ. ID No's:
- 40-42;
- and optionally SEQ. ID No's:
- 49-51
- (m) SEQ. ID No's:
- 43-45;
- and optionally SEQ. ID No's:
- 49-51
- (n) SEQ. ID No's:
- 46-48;
- and optionally SEQ. ID No's:
- 49-51
- Wherein SEQ ID No's:
- 3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48 and 51 are labelled with a quencher and dye, one at the 5′ end and one at the 3′ end.
55 more elements
Dependencies are inferred from the claim text.