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US 10,947,601 B2Granted
Detection of saxitoxin-producing dinoflagellates
AssigneesEwsouth Innovations Pty Limited, Universitetet I Oslo
InventorsBrett A. Neilan, Shauna Ann Murray, Anke Stuken, Kjetill S. Jakobsen, Russel J. S. Orr, Ralf Kellmann
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Abstract
The invention generally relates to the field of saxitoxins and the identification of microorganisms capable of producing them. More specifically, the invention relates to the identification of genes encoding saxitoxin in dinoflagellates, and methods for the specific detection of dinoflagellates that are producers of saxitoxins.
Claims8
A method comprising:
- obtaining a sample for use in the method;
- and
- detecting whether a saxitoxin-producing dinoflagellate is present in the sample by contacting the sample with a nucleic acid primer specific for a dinoflagellate saxitoxin A catalytic domain polynucleotide sequence or an antibody specific for a dinoflagellate saxitoxin A catalytic domain polypeptide, wherein the saxitoxin A catalytic domain is selected from:
- saxitoxin A1 catalytic domain, saxitoxin A4 catalytic domain, or a fragment of a saxitoxin A1 or A4 catalytic domain, and
- wherein:
- the saxitoxin A4 catalytic domain sequence consists of a nucleotide sequence having 95% or more similarity with nucleotides 3115-4121 of the polynucleotide sequence set forth in SEQ ID NO:
- 3, or a nucleotide sequence having 95% or more similarity with nucleotides 3597-3721 of the polynucleotide sequence set forth in SEQ ID NO:
- 3, and
- the saxitoxin A1 catalytic domain sequence consists of a nucleotide sequence having 95% or more similarity with nucleotides 160-1821 of the polynucleotide sequence set forth in SEQ ID NO:
- 1, or a nucleotide sequence having 95% or more similarity with nucleotides 277-2022 of the polynucleotide sequence set forth in SEQ ID NO:
- 3.
8 more elements
Dependencies are inferred from the claim text.