Additive composition used in LAMP reaction
AssigneeDelta Electronics Int'l (Singapore) Pte Ltd
InventorsJunxin Liang, Casthri Krishnamurthy, Shuwen An, Kah Sin Loh, Weishi Zhang
Life of this patent
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Abstract
An additive composition used in a loop mediated isothermal amplification (LAMP) reaction for reducing a threshold time for a positive sample includes but not limited to EDTA, EGTA, BSA, DMSO, nonionic surfactants, and polymers, and the additive composition used in LAMP reactions includes at least one of the above mentioned additives and may be any combination use of the additives.
Claims29
A reaction mixture comprising reaction components for performing a standard loop mediated isothermal amplification (LAMP) reaction and an additional LAMP-specific additive composition for reducing a threshold time for a positive sample and reducing template-independent background amplification, wherein the LAMP-specific additive composition comprises:
- a first additive selected from the group consisting of ethylenediaminetetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA) and a combination thereof;
- and
- a second additive selected from the group consisting of bovine serum albumin (BSA), dimethyl sulfoxide (DMSO), nonionic surfactants, polymers and a combination thereof.
A reaction mixture comprising reaction components for performing a standard loop mediated isothermal amplification (LAMP) reaction and an additional LAMP-specific additive composition for reducing a threshold time for a positive sample and reducing template-independent background amplification, wherein the LAMP-specific additive composition is selected from the group consisting of:
- a first composition comprising a first additive selected from the group consisting of polyethylene glycol (PEG), polyvinylpyrrolidone (PVP), Dextran, Ficoll and a combination thereof, and a second additive selected from the group consisting of dimethyl sulfoxide (DMSO), nonionic surfactants and a combination thereof;
- and
- a second composition comprising a first additive selected from the group consisting of polyethylene glycol (PEG), Dextran, Ficoll and a combination thereof, and a second additive selected from the group consisting of bovine serum albumin (BSA), dimethyl sulfoxide (DMSO), nonionic surfactants and a combination thereof.
A reaction mixture comprising reaction components for performing a standard loop mediated isothermal amplification (LAMP) reaction and an additional LAMP-specific additive composition for reducing a threshold time for a positive sample and reducing template-independent background amplification, wherein the LAMP-specific additive composition consists of a single additive selected from the group consisting of ethylene glycol tetraacetic acid (EGTA), polyvinylpyrrolidone (PVP), Dextran, Ficoll and Tween-80.
Dependencies are inferred from the claim text.