Materials and methods utilizing biotin producing mutant hosts for the production of 7-carbon chemicals

A method for regulating biosynthesis of at least one C7 building block chosen from pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine, 7-aminoheptanol, and 1,7-heptanediol in a recombinant host using a pathway having a pimeloyl-ACP intermediate, said method comprising:

• deleting the activity of an ACP-dependent BioF enzyme;
• overexpressing a BioW enzyme and a CoA-specific BioF enzyme, wherein:
• the BioW enzyme is a pimeloyl-CoA ligase having at least 85% sequence identity or homology to the amino acid sequence set forth in SEQ ID NO:
• 18;
• and
• the CoA-specific BioF enzyme is an 8-amino-7-oxononanoate synthase having at least 85% sequence identity or homology to the amino acid sequence set forth in SEQ ID NO:
• 19;
• and
• wherein the recombinant host is a prokaryote chosen from the genera Escherichia, Clostridia, Corynebacteria, Cupriavidus, Pseudomonas, Delftia, Bacillus, Lactobacillus, Lactococcus, and Rhodococcus or the recombinant host is a eukaryote chosen from the genera Aspergillus, Saccharomyces, Pichia, Yarrowia, Issatchenkia, Debaryomyces, Arxula, and Kluyveromyces;
• and
• wherein the deletion of the activity of an ACP-dependent BioF enzyme occurs through a knockout of the BioF gene.